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Literature summary extracted from

  • Aoki, K.; Shinke, R.; Nishira, H.
    Purification and some properties of yeast tannase (1976), Agric. Biol. Chem., 40, 79-85.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 EDTA 95% remaining activity after 3 days at a concentration of 10 mM, pH 7.2, 0.1 M phosphate buffer Candida sp. (in: Saccharomycetales)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Ca2+ Ca(CH3COO)2: no effect at a concentration of 45 mM at 30°C Candida sp. (in: Saccharomycetales)
3.1.1.20 Co2+ CoCl2: no effect at a concentration of 45 mM at 30°C Candida sp. (in: Saccharomycetales)
3.1.1.20 Cu2+ CuSO4: no effect at a concentration of 45 mM at 30°C Candida sp. (in: Saccharomycetales)
3.1.1.20 Mg2+ MgCl2, MgSO4: no effect at a concentration of 45 mM at 30°C Candida sp. (in: Saccharomycetales)
3.1.1.20 Ni2+ NiSO4, no effect at a concentration of 45 mM at 30°C Candida sp. (in: Saccharomycetales)
3.1.1.20 Zn2+ no effect of ZnCl2 and ZnSO4 at a concentration of 45 mM at 30°C Candida sp. (in: Saccharomycetales)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Candida sp. (in: Saccharomycetales)
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Candida sp. (in: Saccharomycetales)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 613fold Candida sp. (in: Saccharomycetales)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 92
-
 8 step purified enzyme Candida sp. (in: Saccharomycetales)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + H2O
-
 Candida sp. (in: Saccharomycetales) gallic acid + ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Candida sp. (in: Saccharomycetales) 10 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Candida sp. (in: Saccharomycetales) ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 yeast tannase
-
 Candida sp. (in: Saccharomycetales)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 50
-
-
 Candida sp. (in: Saccharomycetales)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 40
-
 stable up to Candida sp. (in: Saccharomycetales)
3.1.1.20 70
-
 rapidly modified and loss of activity Candida sp. (in: Saccharomycetales)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 6
-
-
 Candida sp. (in: Saccharomycetales)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 3.5 9 pH 3.5: 80% of the maximal activity, pH 7.5: 40% of the maximal activity Candida sp. (in: Saccharomycetales)