Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cole, P.W.; Murakami, K.; Inagami, T.
    Specificity and mechanism of clostripain catalysis (1971), Biochemistry, 10, 4246-4252.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.8 benzamidine
-
 Hathewaya histolytica
3.4.22.8 Butylamine
-
 Hathewaya histolytica
3.4.22.8 butylguanidine
-
 Hathewaya histolytica
3.4.22.8 ethylamine
-
 Hathewaya histolytica
3.4.22.8 Ethylguanidine
-
 Hathewaya histolytica
3.4.22.8 methylamine
-
 Hathewaya histolytica
3.4.22.8 methylguanidine
-
 Hathewaya histolytica
3.4.22.8 N-Alpha-p-tosyl-L-homoarginine methyl ester
-
 Hathewaya histolytica
3.4.22.8 N-Alpha-p-tosyl-L-norarginine methyl ester
-
 Hathewaya histolytica
3.4.22.8 Propylamine
-
 Hathewaya histolytica
3.4.22.8 propylguanidine
-
 Hathewaya histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.8 additional information
-
 additional information effect of pH-value on Km-value for the hydrolysis of alpha-benzoyl-Arg ethyl ester, effect of temperature on Km-values Hathewaya histolytica
3.4.22.8 0.022
-
 p-toluenesulfonyl-L-Arg methyl ester
-
 Hathewaya histolytica
3.4.22.8 0.24 0.25 alpha-benzoyl-Arg ethyl ester depending on assay method Hathewaya histolytica
3.4.22.8 0.25
-
 Nalpha-p-tosyl-L-Arg amide
-
 Hathewaya histolytica
3.4.22.8 0.26
-
 p-Nitrophenyl p-guanidinobenzoate
-
 Hathewaya histolytica
3.4.22.8 0.8
-
 p-toluenesulfonyl-L-Arg methyl ester
-
 Hathewaya histolytica
3.4.22.8 1.3
-
 N-benzoyl-L-Arg amide
-
 Hathewaya histolytica
3.4.22.8 3
-
 Nalpha-benzoyl-L-Lys methyl ester
-
 Hathewaya histolytica

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.8 Hathewaya histolytica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.8 N-benzoyl-Arg amide + H2O
-
 Hathewaya histolytica N-benzoyl-Arg + NH3
-
 ?
3.4.22.8 N-benzoyl-Arg ethyl ester + H2O
-
 Hathewaya histolytica N-benzoyl-Arg + ethanol
-
 ?
3.4.22.8 Nalpha-benzoyl-Arg p-nitroanilide + H2O
-
 Hathewaya histolytica Nalpha-benzoyl-Arg + p-nitroaniline
-
 ?
3.4.22.8 Nalpha-benzoyl-L-Lys methyl ester + H2O
-
 Hathewaya histolytica Nalpha-benzoyl-L-Lys + methanol
-
 ?
3.4.22.8 Nalpha-benzoyl-Lys methyl ester + H2O
-
 Hathewaya histolytica Nalpha-benzoyl-Lys + methanol
-
 ?
3.4.22.8 Nalpha-p-tosyl-Arg amide + H2O
-
 Hathewaya histolytica Nalpha-p-tosyl-Arg + NH3
-
 ?
3.4.22.8 p-nitrophenyl p-guanidinobenzoate + H2O
-
 Hathewaya histolytica p-nitrophenol + p-guanidinobenzoate
-
 ?
3.4.22.8 p-tosyl-Arg methyl ester + H2O
-
 Hathewaya histolytica p-tosyl-Arg + methanol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.8 additional information
-
 additional information effect of pH-value on the turnover-number for hydrolysis of alpha-benzoyl-Arg ethyl ester, effect of temperature on turnover numbers Hathewaya histolytica
3.4.22.8 9.8
-
 Nalpha-p-tosyl-L-Arg methyl ester
-
 Hathewaya histolytica
3.4.22.8 10
-
 Nalpha-p-tosyl-L-Arg amide
-
 Hathewaya histolytica
3.4.22.8 22
-
 Nalpha-benzoyl-L-Lys methyl ester
-
 Hathewaya histolytica
3.4.22.8 31
-
 p-Nitrophenyl p-guanidinobenzoate
-
 Hathewaya histolytica
3.4.22.8 86
-
 p-toluenesulfonyl-L-Arg methyl ester
-
 Hathewaya histolytica
3.4.22.8 87
-
 N-benzoyl-L-Arg amide
-
 Hathewaya histolytica
3.4.22.8 91 92 alpha-benzoyl-Arg ethyl ester depending on assay method Hathewaya histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.8 7
-
 hydrolysis of alpha-benzoyl-Arg ethyl ester Hathewaya histolytica