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Literature summary extracted from

  • Nagasawa, S.; Kobayashi, C.; Maki-Suzuki, T.; Yamashita, N.; Koyama, J.
    Purification and characterization of the C3 convertase of the classical pathway of human complement system by size exclusion high-performance liquid chromatography (1985), J. Biochem., 97, 493-499.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.43 complement component C2b accelerates dissociation Homo sapiens
3.4.21.43 complex C4bC2a extremely unstable Homo sapiens
3.4.21.43 dilute iodine solution stabilizes Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.43 Iodine stabilization Homo sapiens
3.4.21.43 Mg2+ formation of the bimolecular complex C4b2a, i.e. C3 convertase Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.43 280000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.43 complement component C3 + H2O Homo sapiens classical pathway of the complement system ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.43 Homo sapiens
-
 2 distinct C3 convertases generated by 2 seperate enzyme cascade systems
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.43
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.43 Selective cleavage of Arg-/-Ser bond in complement component C3 alpha-chain to form C3a and C3b, and Arg-/- bond in complement component C5 alpha-chain to form C5a and C5b hydrolyses peptide bond 77 (Arg-Ser) in complement component C3, and complement component C5. Cleavage of C5 requires complement fragment C3b which binds C5 and renders it susceptible to cleavage by the C4b,C2a complex Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.43 plasma
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.4.21.43 0°C, 8 d, 40% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.43 complement component C3 + H2O hydrolyzes peptide bond Arg77-Ser of the alpha-chain of C3 Homo sapiens complement component C3a + complement component C3b
-
 ?
3.4.21.43 complement component C3 + H2O classical pathway of the complement system Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.43 37
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.43 24
-
 half-life: 400 min Homo sapiens
3.4.21.43 30
-
 half-life: 180 min Homo sapiens
3.4.21.43 37
-
 half-life: 60 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.43 7.5
-
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.43 6.5 9 at pH 6.5 more stable than at pH 9 Homo sapiens