BRENDA - Enzyme Database

Purification and substrate specificity of bovine angiotensin-converting enzyme

Rohrbach, M.S.; Williams, E.B.; Rolstad, R.A.; J. Biol. Chem. 256, 225-230 (1981) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.15.1
0.12
-
benzoyl-Gly-His-Arg
-
Bos taurus
3.4.15.1
0.89
-
benzoyl-Gly-Phe-His-Leu
-
Bos taurus
3.4.15.1
1.2
-
benzoyl-Gly-His-Leu
-
Bos taurus
3.4.15.1
1.3
-
benzoyl-Gly-Arg-Leu
-
Bos taurus
3.4.15.1
2.1
-
benzoyl-Gly-Ala-Leu
-
Bos taurus
3.4.15.1
2.1
-
benzoyl-Gly-Phe-Leu
-
Bos taurus
3.4.15.1
2.3
-
benzoyl-Gly-Ser-His-Leu
-
Bos taurus
3.4.15.1
2.7
-
benzoyl-Gly-Arg-His-Leu
-
Bos taurus
3.4.15.1
3.4
-
benzoyl-Gly-Ala-His-Leu
-
Bos taurus
3.4.15.1
4.4
-
benzoyl-Gly-Ile-His-Leu
-
Bos taurus
3.4.15.1
4.6
-
benzoyl-Gly-Pro-His-Leu
-
Bos taurus
3.4.15.1
4.9
-
benzoyl-Gly-Glu-Leu
-
Bos taurus
3.4.15.1
4.9
-
benzoyl-Gly-His-Ala
-
Bos taurus
3.4.15.1
5.2
-
benzoyl-Gly-His-Phe
-
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.15.1
126000
-
x * 126000, denatured and reduced denatured enzyme, SDS-PAGE
Bos taurus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.15.1
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.15.1
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.15.1
lung
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.15.1
additional information
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.15.1
benzoyl-Gly-Ala-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Ala + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Ala-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + Ala-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Arg-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Arg + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Arg-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + Arg-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Glu-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + Glu-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-His-Ala + H2O
-
81271
Bos taurus
benzoyl-Gly + His-Ala
-
-
-
?
3.4.15.1
benzoyl-Gly-His-Arg + H2O
-
81271
Bos taurus
benzoyl-Gly + His-Arg
-
-
-
?
3.4.15.1
benzoyl-Gly-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-His-Phe + H2O
-
81271
Bos taurus
benzoyl-Gly + His-Phe
-
-
-
?
3.4.15.1
benzoyl-Gly-Ile-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Ile + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Phe-Arg + H2O
-
81271
Bos taurus
benzoyl-Gly + Phe-Arg
-
-
-
?
3.4.15.1
benzoyl-Gly-Phe-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Phe + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Phe-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + Phe-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Pro-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Pro + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Ser-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Ser + His-Leu
-
-
-
?
3.4.15.1
hippuryl-His-Leu + H2O
-
81271
Bos taurus
hippuric acid + His-Leu
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.15.1
?
x * 126000, denatured and reduced denatured enzyme, SDS-PAGE
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.15.1
8.8
-
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.15.1
0.12
-
benzoyl-Gly-His-Arg
-
Bos taurus
3.4.15.1
0.89
-
benzoyl-Gly-Phe-His-Leu
-
Bos taurus
3.4.15.1
1.2
-
benzoyl-Gly-His-Leu
-
Bos taurus
3.4.15.1
1.3
-
benzoyl-Gly-Arg-Leu
-
Bos taurus
3.4.15.1
2.1
-
benzoyl-Gly-Ala-Leu
-
Bos taurus
3.4.15.1
2.1
-
benzoyl-Gly-Phe-Leu
-
Bos taurus
3.4.15.1
2.3
-
benzoyl-Gly-Ser-His-Leu
-
Bos taurus
3.4.15.1
2.7
-
benzoyl-Gly-Arg-His-Leu
-
Bos taurus
3.4.15.1
3.4
-
benzoyl-Gly-Ala-His-Leu
-
Bos taurus
3.4.15.1
4.4
-
benzoyl-Gly-Ile-His-Leu
-
Bos taurus
3.4.15.1
4.6
-
benzoyl-Gly-Pro-His-Leu
-
Bos taurus
3.4.15.1
4.9
-
benzoyl-Gly-Glu-Leu
-
Bos taurus
3.4.15.1
4.9
-
benzoyl-Gly-His-Ala
-
Bos taurus
3.4.15.1
5.2
-
benzoyl-Gly-His-Phe
-
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.15.1
126000
-
x * 126000, denatured and reduced denatured enzyme, SDS-PAGE
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.15.1
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.15.1
lung
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.15.1
additional information
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.15.1
benzoyl-Gly-Ala-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Ala + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Ala-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + Ala-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Arg-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Arg + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Arg-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + Arg-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Glu-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + Glu-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-His-Ala + H2O
-
81271
Bos taurus
benzoyl-Gly + His-Ala
-
-
-
?
3.4.15.1
benzoyl-Gly-His-Arg + H2O
-
81271
Bos taurus
benzoyl-Gly + His-Arg
-
-
-
?
3.4.15.1
benzoyl-Gly-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-His-Phe + H2O
-
81271
Bos taurus
benzoyl-Gly + His-Phe
-
-
-
?
3.4.15.1
benzoyl-Gly-Ile-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Ile + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Phe-Arg + H2O
-
81271
Bos taurus
benzoyl-Gly + Phe-Arg
-
-
-
?
3.4.15.1
benzoyl-Gly-Phe-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Phe + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Phe-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly + Phe-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Pro-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Pro + His-Leu
-
-
-
?
3.4.15.1
benzoyl-Gly-Ser-His-Leu + H2O
-
81271
Bos taurus
benzoyl-Gly-Ser + His-Leu
-
-
-
?
3.4.15.1
hippuryl-His-Leu + H2O
-
81271
Bos taurus
hippuric acid + His-Leu
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.15.1
?
x * 126000, denatured and reduced denatured enzyme, SDS-PAGE
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.15.1
8.8
-
-
Bos taurus