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Literature summary extracted from
- Extracellular aminopeptidase from Clostridium histolyticum (1976), Methods Enzymol., 45, 544-552.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.11.13 | very sensitive to traces of Zn2+ | Hathewaya histolytica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.13 | Cu2+ | - |
Hathewaya histolytica |  |
| 3.4.11.13 | p-mercuribenzoate | - |
Hathewaya histolytica | |
| 3.4.11.13 | Zn2+ | - |
Hathewaya histolytica | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.13 | 6.45 | - |
Leu-Gly-Gly | - |
Hathewaya histolytica | |
| 3.4.11.13 | 8.4 | - |
Phe-Gly-Gly | - |
Hathewaya histolytica | |
| 3.4.11.13 | 18.5 | - |
Val-Gly-Gly | - |
Hathewaya histolytica | |
| 3.4.11.13 | 20 | - |
Pro-Gly-Pro | - |
Hathewaya histolytica | |
| 3.4.11.13 | 20 | - |
Pro-Gly-Pro-Pro | - |
Hathewaya histolytica | |
| 3.4.11.13 | 40 | - |
Pro-Gly-Gly | - |
Hathewaya histolytica | |
| 3.4.11.13 | 80 | - |
Ala-Gly-Gly | - |
Hathewaya histolytica | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.13 | extracellular | - |
Hathewaya histolytica | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.13 | Cd2+ | - |
Hathewaya histolytica | |
| 3.4.11.13 | Co2+ | optiumum concentration 0.025 mM | Hathewaya histolytica | |
| 3.4.11.13 | Mn2+ | best activator, optimum concentration 0.05 mM | Hathewaya histolytica | |
| 3.4.11.13 | Ni2+ | - |
Hathewaya histolytica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.13 | 340000 | - |
gel filtration | Hathewaya histolytica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.13 | Peptides + H2O | Hathewaya histolytica | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.13 | Hathewaya histolytica | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.13 | - |
Hathewaya histolytica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.13 | 10.5 | - |
- |
Hathewaya histolytica |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.11.13 | -20°C, 0.05 M phosphate buffer or 0.05 M Na-acetate buffer, pH 5.6, 1 mM EDTA, several years, little loss of activity, can be restored by treatment with 2.5 mM dithiothreitol | Hathewaya histolytica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.13 | (Ala)3 + H2O | - |
Hathewaya histolytica | Ala + Ala-Ala | - |
ir | |
| 3.4.11.13 | (Ala)4 + H2O | - |
Hathewaya histolytica | Ala + (Ala)3 | or Ala-Ala + Ala-Ala | ir | |
| 3.4.11.13 | (Ala)4-NH2 + H2O | - |
Hathewaya histolytica | Ala + (Ala)3-NH2 | or Ala-Ala + Ala-Ala-NH2 or (Ala)3 + Ala-NH2 | ir | |
| 3.4.11.13 | (Pro-Gly-Pro)4-6 + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro-(Pro-Gly-Pro)3-5 | - |
ir | |
| 3.4.11.13 | (Pro-Gly-Pro)4-6-OMe + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro-(Pro-Gly-Pro)3-5-OMe | - |
ir | |
| 3.4.11.13 | Ala-Ala-Phe-Ala + H2O | - |
Hathewaya histolytica | Ala + Ala-Phe-Ala | or Ala-Ala + Phe-Ala | ir | |
| 3.4.11.13 | Ala-Gly-Gly + H2O | - |
Hathewaya histolytica | Ala + Gly-Gly | - |
ir | |
| 3.4.11.13 | Ala-Lys + H2O | - |
Hathewaya histolytica | Ala + Lys | - |
ir | |
| 3.4.11.13 | Gly-Gly-Phe + H2O | - |
Hathewaya histolytica | Gly + Gly-Phe | - |
ir | |
| 3.4.11.13 | Gly-Gly-Phe-Ala + H2O | - |
Hathewaya histolytica | Gly + Gly-Phe-Ala | or Gly-Gly + Phe-Ala | ir | |
| 3.4.11.13 | Gly-Phe-Ala + H2O | - |
Hathewaya histolytica | Gly + Phe-Ala | - |
ir | |
| 3.4.11.13 | Hyp-Ala-NH2 + H2O | - |
Hathewaya histolytica | Hyp-Ala + NH3 | - |
ir | |
| 3.4.11.13 | Leu-Gly-Gly + H2O | - |
Hathewaya histolytica | Leu + Gly-Gly | - |
ir | |
| 3.4.11.13 | Met-Gly-Gly + H2O | - |
Hathewaya histolytica | Met + Gly-Gly | - |
ir | |
| 3.4.11.13 | Met-Met + H2O | - |
Hathewaya histolytica | Met + Met | - |
ir | |
| 3.4.11.13 | Peptides + H2O | - |
Hathewaya histolytica | ? | - |
? | |
| 3.4.11.13 | Phe-Gly-Gly + H2O | - |
Hathewaya histolytica | Phe + Gly-Gly | - |
ir | |
| 3.4.11.13 | Pro-Gly-Gly + H2O | - |
Hathewaya histolytica | Pro + Gly-Gly | - |
ir | |
| 3.4.11.13 | Pro-Gly-Pro + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro | - |
? | |
| 3.4.11.13 | Pro-Gly-Pro-Pro + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro-Pro | - |
ir | |
| 3.4.11.13 | Pro-Leu + H2O | - |
Hathewaya histolytica | Pro + Leu | - |
ir | |
| 3.4.11.13 | Pro-Phe + H2O | - |
Hathewaya histolytica | Pro + Phe | - |
ir | |
| 3.4.11.13 | Ser-Gly-Gly + H2O | - |
Hathewaya histolytica | Ser + Gly-Gly | - |
ir | |
| 3.4.11.13 | Val-Gly-Gly + H2O | - |
Hathewaya histolytica | Val + Gly-Gly | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.13 | hexamer | 6 * 51000-59000, SDS-PAGE | Hathewaya histolytica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.13 | 40 | - |
assay at | Hathewaya histolytica |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.13 | 276 | - |
Val-Gly-Gly | - |
Hathewaya histolytica | |
| 3.4.11.13 | 337 | - |
Pro-Gly-Pro-Pro | - |
Hathewaya histolytica | |
| 3.4.11.13 | 561 | - |
Pro-Gly-Pro | - |
Hathewaya histolytica | |
| 3.4.11.13 | 667 | - |
Phe-Gly-Gly | - |
Hathewaya histolytica | |
| 3.4.11.13 | 917 | - |
Leu-Gly-Gly | - |
Hathewaya histolytica | |
| 3.4.11.13 | 1360 | - |
Pro-Gly-Gly | - |
Hathewaya histolytica | |
| 3.4.11.13 | 1830 | - |
Ala-Gly-Gly | - |
Hathewaya histolytica | |
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