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Literature summary extracted from

  • Norbeck, J.; Pahlman, A.; Akhtar, N.; Blomberg, A.; Adler, L.
    Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-acti (1996), J. Biol. Chem., 271, 13875-1388.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.21 3.1
-
 DL-glycerol-3-phosphate for Gpp1p Saccharomyces cerevisiae
3.1.3.21 3.9
-
 DL-glycerol-3-phosphate for Gpp2p Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.21 cytosol strict specificity avoids interference with plethora of phosphorylated metabolites in the metabolic network Saccharomyces cerevisiae 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.21 27800
-
 Gpp2p, calculation from sequence of cDNA Saccharomyces cerevisiae
3.1.3.21 30400
-
 Gpp1p, calculation from sequence of cDNA Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.21 glycerol-3-phosphate + H2O Saccharomyces cerevisiae glycerol metabolism glycerol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.21 Saccharomyces cerevisiae
-
 baker`s yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.21 gel filtration and ion exchange chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.21 46
-
 enzyme Gpp2p Saccharomyces cerevisiae
3.1.3.21 49
-
 enzyme Gpp1p Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.21 DL-glycerol-3-phosphate + H2O no stereospecificity Saccharomyces cerevisiae glycerol + phosphate
-
 ?
3.1.3.21 glycerol-3-phosphate + H2O glycerol metabolism Saccharomyces cerevisiae glycerol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.21 monomer gel filtration and non-denaturating gradient gel electrophoresis Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.21 6.5
-
 enzymes Gpp1p and Gpp2p Saccharomyces cerevisiae