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Literature summary extracted from

  • Yuan, L.; Nelson, B.A.; Caryl, G.
    The catalytic cysteine and histidine in the plant acyl-acyl carrier protein thioesterases (1996), J. Biol. Chem., 271, 3417-3419.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 expression of UcFatB1 and mutants in Escherichia coli Umbellularia californica

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.14 C320A no activity Umbellularia californica
3.1.2.14 C320S slightly reduced activity Umbellularia californica
3.1.2.14 H140A reduced activity Umbellularia californica
3.1.2.14 H140R reduced activity Umbellularia californica
3.1.2.14 H285A no activity Umbellularia californica
3.1.2.14 H285R reduced activity Umbellularia californica
3.1.2.14 H345A reduced activity Umbellularia californica
3.1.2.14 H345R reduced activity Umbellularia californica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.14 5,5'-dithiobis(2-nitrobenzoate)
-
 Umbellularia californica
3.1.2.14 diethyldicarbonate
-
 Umbellularia californica
3.1.2.14 iodoacetamide
-
 Umbellularia californica
3.1.2.14 additional information no inhibition by phenylmethylsulfonylfluoride Umbellularia californica

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Arabidopsis thaliana
-
-
-
3.1.2.14 Brassica napus
-
-
-
3.1.2.14 Carthamus tinctorius
-
-
-
3.1.2.14 Umbellularia californica
-
 bay, UcFatB1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.14 Uc FatB1 and mutants Umbellularia californica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 lauroyl-[acyl-carrier protein] + H2O
-
 Umbellularia californica lauric acid + acyl-carrier protein
-
 ?