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Literature summary extracted from
- Studies on an enzyme, S-formylglutathione hydrolase, of the dissimilatory pathway of methanol in Candida boidinii (1980), Biochim. Biophys. Acta, 614, 81-91.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.2.12 | glutathione | - |
[Candida] boidinii |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.2.12 | 0.21 | - |
S-formylglutathione | - |
[Candida] boidinii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.12 | 25000 | - |
1 * 25000 + 1 * 35000, SDS-PAGE | [Candida] boidinii |
| 3.1.2.12 | 35000 | - |
1 * 25000 + 1 * 35000, SDS-PAGE | [Candida] boidinii |
| 3.1.2.12 | 61000 | 64000 | gel filtration, sedimentation equilibrium centrifugation | [Candida] boidinii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.12 | S-formylglutathione + H2O | [Candida] boidinii | dissimilatory pathway during growth on methanol | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.2.12 | [Candida] boidinii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.2.12 | - |
[Candida] boidinii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.12 | 1350 | - |
- |
[Candida] boidinii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.2.12 | -20°C, 50% glycerol, 6 months | [Candida] boidinii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.12 | S-acetylglutathione + H2O | 1.3% of reaction rate with S-formylglutathione | [Candida] boidinii | glutathione + acetate | - |
? | |
| 3.1.2.12 | S-formylglutathione + H2O | - |
[Candida] boidinii | glutathione + formate | - |
? | |
| 3.1.2.12 | S-formylglutathione + H2O | dissimilatory pathway during growth on methanol | [Candida] boidinii | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.2.12 | dimer | 1 * 25000 + 1 * 35000, SDS-PAGE | [Candida] boidinii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.12 | 40 | - |
unstable | [Candida] boidinii |
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