BRENDA - Enzyme Database show

Substrate specificity of an alpha-amino acid ester hydrolase produced by Acetobacter turbidans A.T.C.C. 9325

Takahashi, T.; Yamazaki, Y.; Kato, K.; Biochem. J. 137, 497-503 (1974)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.43
ampicillin
hydrolysis, substrate inhibition
Acetobacter pasteurianus
3.1.1.43
ethyl D-alpha-aminophenylacetate
hydrolysis, substrate inhibition
Acetobacter pasteurianus
3.1.1.43
methyl D-alpha-aminophenylacetate
hydrolysis, substrate inhibition
Acetobacter pasteurianus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.43
0.5
-
L-S-benzylcysteine ethyl ester
-
Acetobacter pasteurianus
3.1.1.43
0.6
-
ampicillin
-
Acetobacter pasteurianus
3.1.1.43
0.8
-
L-valine methyl ester
-
Acetobacter pasteurianus
3.1.1.43
2.6
-
ethyl DL-alpha-amino-n-butyrate
-
Acetobacter pasteurianus
3.1.1.43
4.3
-
methyl D-alpha-aminophenylacetate
-
Acetobacter pasteurianus
3.1.1.43
5
-
glycine ethyl ester
-
Acetobacter pasteurianus
3.1.1.43
24
-
methyl L-alpha-aminophenylacetate
-
Acetobacter pasteurianus
3.1.1.43
32.2
-
methyl DL-alpha-aminoisobutyrate
-
Acetobacter pasteurianus
3.1.1.43
40.9
-
D-alanine methyl ester
-
Acetobacter pasteurianus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.43
Acetobacter pasteurianus
-
ATCC 9325
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.43
-
Acetobacter pasteurianus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.43
93.5
-
-
Acetobacter pasteurianus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.43
alpha-aminoisobutyric acid methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
alpha-aminoisobutyric acid methyl ester + H2O
-
80936
Acetobacter pasteurianus
alpha-aminoisobutyric acid + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-alanine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-alanine methyl ester + H2O
-
80936
Acetobacter pasteurianus
D-alanine + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-alpha-phenylglycine methyl ester + 7-aminocephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
-
-
?
3.1.1.43
D-alpha-phenylglycine methyl ester + H2O
-
80936
Acetobacter pasteurianus
D-alpha-phenylglycine + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-methionine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-methionine methyl ester + H2O
-
80936
Acetobacter pasteurianus
D-methionine + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
glycine ethyl ester + 7-aminocephalosporanic acid
-
80936
Acetobacter pasteurianus
ethanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
glycine ethyl ester + H2O
-
80936
Acetobacter pasteurianus
glycine + ethanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
L-cysteine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
L-cysteine methyl ester + H2O
-
80936
Acetobacter pasteurianus
L-cysteine + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
L-serine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
L-serine methyl ester + H2O
-
80936
Acetobacter pasteurianus
L-serine + methanol
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.43
ampicillin
hydrolysis, substrate inhibition
Acetobacter pasteurianus
3.1.1.43
ethyl D-alpha-aminophenylacetate
hydrolysis, substrate inhibition
Acetobacter pasteurianus
3.1.1.43
methyl D-alpha-aminophenylacetate
hydrolysis, substrate inhibition
Acetobacter pasteurianus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.43
0.5
-
L-S-benzylcysteine ethyl ester
-
Acetobacter pasteurianus
3.1.1.43
0.6
-
ampicillin
-
Acetobacter pasteurianus
3.1.1.43
0.8
-
L-valine methyl ester
-
Acetobacter pasteurianus
3.1.1.43
2.6
-
ethyl DL-alpha-amino-n-butyrate
-
Acetobacter pasteurianus
3.1.1.43
4.3
-
methyl D-alpha-aminophenylacetate
-
Acetobacter pasteurianus
3.1.1.43
5
-
glycine ethyl ester
-
Acetobacter pasteurianus
3.1.1.43
24
-
methyl L-alpha-aminophenylacetate
-
Acetobacter pasteurianus
3.1.1.43
32.2
-
methyl DL-alpha-aminoisobutyrate
-
Acetobacter pasteurianus
3.1.1.43
40.9
-
D-alanine methyl ester
-
Acetobacter pasteurianus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.43
-
Acetobacter pasteurianus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.43
93.5
-
-
Acetobacter pasteurianus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.43
alpha-aminoisobutyric acid methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
alpha-aminoisobutyric acid methyl ester + H2O
-
80936
Acetobacter pasteurianus
alpha-aminoisobutyric acid + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-alanine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-alanine methyl ester + H2O
-
80936
Acetobacter pasteurianus
D-alanine + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-alpha-phenylglycine methyl ester + 7-aminocephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
-
-
?
3.1.1.43
D-alpha-phenylglycine methyl ester + H2O
-
80936
Acetobacter pasteurianus
D-alpha-phenylglycine + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-methionine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
D-methionine methyl ester + H2O
-
80936
Acetobacter pasteurianus
D-methionine + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
glycine ethyl ester + 7-aminocephalosporanic acid
-
80936
Acetobacter pasteurianus
ethanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
glycine ethyl ester + H2O
-
80936
Acetobacter pasteurianus
glycine + ethanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
L-cysteine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
L-cysteine methyl ester + H2O
-
80936
Acetobacter pasteurianus
L-cysteine + methanol
-
80936
Acetobacter pasteurianus
?
3.1.1.43
L-serine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
80936
Acetobacter pasteurianus
methanol + ?
-
80936
Acetobacter pasteurianus
?
3.1.1.43
L-serine methyl ester + H2O
-
80936
Acetobacter pasteurianus
L-serine + methanol
-
-
-
?