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Literature summary extracted from

  • Nishio, T.; Chikano, T.; Kamimura, M.
    Purification and some properties of lipase produced by Pseudomonas fragi 22.39 B (1987), Agric. Biol. Chem., 51, 181-186.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 polyoxyethylene sorbitan monooleate 0.5%, weak activation Pseudomonas fragi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 cetyl trimethylammonium bromide
-
 Pseudomonas fragi
3.1.1.3 Co2+ 1 mM, 13% inhibition Pseudomonas fragi
3.1.1.3 dodecyltrimethylammonium chloride
-
 Pseudomonas fragi
3.1.1.3 Fe2+ 1 mM, 63% inhibition Pseudomonas fragi
3.1.1.3 Fe3+ 1 mM, 67% inhibition Pseudomonas fragi
3.1.1.3 Hg2+ 1 mM, 16% inhibition Pseudomonas fragi
3.1.1.3 Mn2+ 1 mM, 18% inhibition Pseudomonas fragi
3.1.1.3 n-dodecyltrimethylammonium bromide
-
 Pseudomonas fragi
3.1.1.3 Pb2+ 1 mM, 22% inhibition Pseudomonas fragi
3.1.1.3 polyoxyethylene oleyl ether
-
 Pseudomonas fragi
3.1.1.3 Sn2+ 1 mM, 20% inhibition Pseudomonas fragi
3.1.1.3 Sodium dodecyl sulfate
-
 Pseudomonas fragi
3.1.1.3 sorbitan monooleate
-
 Pseudomonas fragi
3.1.1.3 Zn2+ 1 mM, 65% inhibition Pseudomonas fragi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 33000
-
 x * 33000, SDS-PAGE Pseudomonas fragi

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 Ethanol inactivates at 45°C Pseudomonas fragi
3.1.1.3 Methanol inactivates at 45°C Pseudomonas fragi

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas fragi
-
 22-39 B
-
3.1.1.3 Pseudomonas fragi 22-39 B
-
 22-39 B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3
-
 Pseudomonas fragi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 additional information
-
-
 Pseudomonas fragi

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 33000, SDS-PAGE Pseudomonas fragi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 65
-
-
 Pseudomonas fragi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 40 80 40°C: about 55% of maximal activity, 80°C: about 90% of maximal activity Pseudomonas fragi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 51
-
 pH 9.0, 24 h, stable up to Pseudomonas fragi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 9
-
-
 Pseudomonas fragi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 7 10 pH 7.0: about 55% of maximal activity, pH 10.0: about 40% of maximal activity Pseudomonas fragi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 6.5 10.5 30°C, 24 h, stable Pseudomonas fragi
3.1.1.3 8 10.5 50°C, 24 h Pseudomonas fragi