Any feedback?
Literature summary extracted from
- Purification and some properties of lipase produced by Pseudomonas fragi 22.39 B (1987), Agric. Biol. Chem., 51, 181-186.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | polyoxyethylene sorbitan monooleate | 0.5%, weak activation | Pseudomonas fragi |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | cetyl trimethylammonium bromide | - |
Pseudomonas fragi | |
| 3.1.1.3 | Co2+ | 1 mM, 13% inhibition | Pseudomonas fragi | |
| 3.1.1.3 | dodecyltrimethylammonium chloride | - |
Pseudomonas fragi | |
| 3.1.1.3 | Fe2+ | 1 mM, 63% inhibition | Pseudomonas fragi | |
| 3.1.1.3 | Fe3+ | 1 mM, 67% inhibition | Pseudomonas fragi | |
| 3.1.1.3 | Hg2+ | 1 mM, 16% inhibition | Pseudomonas fragi | |
| 3.1.1.3 | Mn2+ | 1 mM, 18% inhibition | Pseudomonas fragi | |
| 3.1.1.3 | n-dodecyltrimethylammonium bromide | - |
Pseudomonas fragi | |
| 3.1.1.3 | Pb2+ | 1 mM, 22% inhibition | Pseudomonas fragi | |
| 3.1.1.3 | polyoxyethylene oleyl ether | - |
Pseudomonas fragi | |
| 3.1.1.3 | Sn2+ | 1 mM, 20% inhibition | Pseudomonas fragi | |
| 3.1.1.3 | Sodium dodecyl sulfate | - |
Pseudomonas fragi | |
| 3.1.1.3 | sorbitan monooleate | - |
Pseudomonas fragi | |
| 3.1.1.3 | Zn2+ | 1 mM, 65% inhibition | Pseudomonas fragi | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 33000 | - |
x * 33000, SDS-PAGE | Pseudomonas fragi |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | Ethanol | inactivates at 45°C | Pseudomonas fragi |
| 3.1.1.3 | Methanol | inactivates at 45°C | Pseudomonas fragi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Pseudomonas fragi | - |
22-39 B | - |
| 3.1.1.3 | Pseudomonas fragi 22-39 B | - |
22-39 B | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | - |
Pseudomonas fragi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | additional information | - |
- |
Pseudomonas fragi |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | ? | x * 33000, SDS-PAGE | Pseudomonas fragi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 65 | - |
- |
Pseudomonas fragi |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 40 | 80 | 40°C: about 55% of maximal activity, 80°C: about 90% of maximal activity | Pseudomonas fragi |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 51 | - |
pH 9.0, 24 h, stable up to | Pseudomonas fragi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 9 | - |
- |
Pseudomonas fragi |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 7 | 10 | pH 7.0: about 55% of maximal activity, pH 10.0: about 40% of maximal activity | Pseudomonas fragi |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 6.5 | 10.5 | 30°C, 24 h, stable | Pseudomonas fragi |
| 3.1.1.3 | 8 | 10.5 | 50°C, 24 h | Pseudomonas fragi |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
