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Literature summary extracted from

  • Koenig, K.; Andreesen, J.R.
    Molybdenum involvement in aerobic degradation of 2-furoic acid by Pseudomonas putida Fu1 (1989), Appl. Environ. Microbiol., 55, 1829-1834.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.8 cyanide
-
 Pseudomonas putida
1.3.99.8 tungstate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.8 0.02 0.05 2-Furoyl-CoA
-
 Pseudomonas putida
6.2.1.31 0.75
-
 2-furoate
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.99.8 Molybdenum
-
 Pseudomonas putida
6.2.1.31 Mg2+ requirement Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.8 100000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.8 2-furoyl-CoA + H2O + acceptor Pseudomonas putida
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor Pseudomonas putida Fu1
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.8 Pseudomonas putida
-
-
-
1.3.99.8 Pseudomonas putida Fu1
-
-
-
6.2.1.31 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.31 strain F1, partial Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.8 0.09
-
 micromol 2-furoyl-CoA/min/mg, in crude extract Pseudomonas putida
6.2.1.31 0.2
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
6.2.1.31 -18°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.8 2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida Fu1 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida Fu1 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
6.2.1.31 ATP + 2-furoate + CoA
-
 Pseudomonas putida AMP + diphosphate + 2-furoyl-CoA
-
 ?
6.2.1.31 ATP + 2-furoate + CoA
-
 Pseudomonas putida Fu1 AMP + diphosphate + 2-furoyl-CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.31 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.8 7.5
-
 2-furoyl-CoA + nitro blue tetrazolium Pseudomonas putida
6.2.1.31 8.5 9.5 broad Pseudomonas putida