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Literature summary extracted from

  • Kuo, E.Y.; Cai, M.S.; Lee, T.M.
    Ascorbate peroxidase 4 plays a role in the tolerance of Chlamydomonas reinhardtii to photo-oxidative stress (2020), Sci. Rep., 10, 13287 .
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.11 Fe2+ in the heme group Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.11 2 L-ascorbate + H2O2 + 2 H+ Chlamydomonas reinhardtii
-
 L-ascorbate + L-dehydroascorbate + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Chlamydomonas reinhardtii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 2 L-ascorbate + H2O2 + 2 H+
-
 Chlamydomonas reinhardtii L-ascorbate + L-dehydroascorbate + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.11 APX4
-
 Chlamydomonas reinhardtii
1.11.1.11 ascorbate peroxidase 4
-
 Chlamydomonas reinhardtii
1.11.1.11 CrAPX4
-
 Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.11 heme
-
 Chlamydomonas reinhardtii

Expression

EC Number Organism Comment Expression
1.11.1.11 Chlamydomonas reinhardtii CrAPX4 is downregulated using amiRNA technology to examine the role of APX for high-light (HL) acclimation. The CrAPX4 knockdown amiRNA lines show low APX activity and CrAPX4 transcript level without a change in CrAPX1 and CrAPX2 transcript levels, and monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) activities and transcript levels. Upon exposure to high-light (HL), CrAPX4 knockdown amiRNA lines show a modification in the expression of genes encoding the enzymes in the ascorbate-glutathione cycle, including an increase in transcript level of CrVTC2, a key enzyme for ascorbate (AsA) biosynthesis but a decrease in MDAR and DHAR transcription and activity after 1 h, followed by increases in reactive oxygen species production and lipid peroxidation after 6 h, and exhibit cell death after 9 h. Besides, AsA content and AsA/DHA (dehydroascorbate) ratio decrease in CrAPX4 knockdown amiRNA lines after prolonged HL treatment additional information

General Information

EC Number General Information Comment Organism
1.11.1.11 malfunction CrAPX4 knockdown amiRNA lines show low APX activity and CrAPX4 transcript level without a change in CrAPX1 and CrAPX2 transcript levels, and monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) activities and transcript levels. Upon exposure to high-light (HL), CrAPX4 knockdown amiRNA lines show a modification in the expression of genes encoding the enzymes in the ascorbate-glutathione cycle, including an increase in transcript level of CrVTC2, a key enzyme for ascorbate (AsA) biosynthesis but a decrease in MDAR and DHAR transcription and activity after 1 h, followed by increases in reactive oxygen species production and lipid peroxidation after 6 h, and exhibit cell death after 9 h. Besides, AsA content and AsA/DHA (dehydroascorbate) ratio decrease in CrAPX4 knockdown amiRNA lines after prolonged HL treatment Chlamydomonas reinhardtii
1.11.1.11 physiological function isozyme CrAPX4 induction together with its association with the modulation of MDAR and DHAR expression for AsA regeneration is critical for Chlamydomonas to cope with photooxidative stress. APX is a central enzyme for ROS scavenging in plants can be induced under abiotic and biotic stresses Chlamydomonas reinhardtii