Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sharma, M.; Lingford, J.P.; Petricevic, M.; Snow, A.J.D.; Zhang, Y.; Jaerva, M.A.; Mui, J.W.; Scott, N.E.; Saunders, E.C.; Mao, R.; Epa, R.; da Silva, B.M.; Pires, D.E.V.; Ascher, D.B.; McConville, M.J.; Davies, G.J.; Williams, S.J.; Goddard-Borger, E.D.
    Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria (2022), Proc. Natl. Acad. Sci. USA, 119, e2116022119 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.432 in complex with NADPH and as ternary product complex by soaking crystals with D-glucose, to 1.5 A resolution. SmoB is an (alpha/beta)8 TIM barrel fold with a C-terminal cofactor binding site. Glucose interacts with residues Arg152 and Lys120 as well as His151 and Tyr76 within the conserved catalytic tetrad His/Tyr/Lys/Asp. SmoB exists as a compact trimer Agrobacterium fabrum
1.14.14.181 to 1.9 A resolution. Enzyme consists of a core (alpha/beta)8 triose-phosphate isomerase barrel with three additional insertion regions Rhizobium oryzae
1.14.14.181 to 3.4 A resolution Agrobacterium fabrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.432 D-glucose + NADP+ Agrobacterium fabrum
-
 6-dehydro-D-glucose + NADPH + H+
-
 ?
1.1.1.432 D-glucose + NADP+ Agrobacterium fabrum ATCC 33970
-
 6-dehydro-D-glucose + NADPH + H+
-
 ?
1.14.14.181 6-sulfo-D-quinovose + FMNH2 + O2 Agrobacterium fabrum
-
 6-dehydro-D-glucose + FMN + sulfite + H2O
-
 ?
1.14.14.181 6-sulfo-D-quinovose + FMNH2 + O2 Rhizobium oryzae
-
 6-dehydro-D-glucose + FMN + sulfite + H2O
-
 ?
1.14.14.181 6-sulfo-D-quinovose + FMNH2 + O2 Agrobacterium fabrum C58
-
 6-dehydro-D-glucose + FMN + sulfite + H2O
-
 ?
1.14.14.181 additional information Agrobacterium fabrum enzyme is highly specific for 6-sulfo-D-quinovose. 6-Sulfo-D-quinovose binds SmoC with Kd of 3 microM. No activity with other sulfonates tested ?
-
-
1.14.14.181 additional information Agrobacterium fabrum C58 enzyme is highly specific for 6-sulfo-D-quinovose. 6-Sulfo-D-quinovose binds SmoC with Kd of 3 microM. No activity with other sulfonates tested ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.432 Agrobacterium fabrum A9CEY6
-
-
1.1.1.432 Agrobacterium fabrum ATCC 33970 A9CEY6
-
-
1.14.14.181 Agrobacterium fabrum A9CEY7
-
-
1.14.14.181 Agrobacterium fabrum C58 A9CEY7
-
-
1.14.14.181 Rhizobium oryzae A0A1X7D696
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.432 D-glucose + NADP+
-
 Agrobacterium fabrum 6-dehydro-D-glucose + NADPH + H+
-
 ?
1.1.1.432 D-glucose + NADP+
-
 Agrobacterium fabrum ATCC 33970 6-dehydro-D-glucose + NADPH + H+
-
 ?
1.14.14.181 6-sulfo-D-quinovose + FMNH2 + O2
-
 Agrobacterium fabrum 6-dehydro-D-glucose + FMN + sulfite + H2O
-
 ?
1.14.14.181 6-sulfo-D-quinovose + FMNH2 + O2
-
 Rhizobium oryzae 6-dehydro-D-glucose + FMN + sulfite + H2O
-
 ?
1.14.14.181 6-sulfo-D-quinovose + FMNH2 + O2
-
 Agrobacterium fabrum C58 6-dehydro-D-glucose + FMN + sulfite + H2O
-
 ?
1.14.14.181 additional information enzyme is highly specific for 6-sulfo-D-quinovose. 6-Sulfo-D-quinovose binds SmoC with Kd of 3 microM. No activity with other sulfonates tested Agrobacterium fabrum ?
-
-
1.14.14.181 additional information enzyme is highly specific for 6-sulfo-D-quinovose. 6-Sulfo-D-quinovose binds SmoC with Kd of 3 microM. No activity with other sulfonates tested Agrobacterium fabrum C58 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.1.432 trimer crystallization data Agrobacterium fabrum
1.14.14.181 dimer crystallization data Agrobacterium fabrum
1.14.14.181 dimer crystallization data Rhizobium oryzae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.432 Atu3278
-
 Agrobacterium fabrum
1.1.1.432 smoB
-
 Agrobacterium fabrum
1.14.14.181 SAMN02982989_5106
-
 Rhizobium oryzae
1.14.14.181 smoC
-
 Agrobacterium fabrum
1.14.14.181 smoC
-
 Rhizobium oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.432 additional information NAD+ does not support the reaction, no binding is observed for NADH Agrobacterium fabrum
1.1.1.432 NADP+
-
 Agrobacterium fabrum
1.1.1.432 NADPH binds to SmoB with Kd of about 2 microM Agrobacterium fabrum
1.14.14.181 FMN FMN binding site is a deep hydrophobic pocket that accommodates the isoalloxazine ring system and extends out to the protein-solvent interface, which is gated by conserved phosphate-binding residues Tyr136 and Ser189 Rhizobium oryzae

General Information

EC Number General Information Comment Organism
1.1.1.432 evolution the sulfoquinovose monooxygenase pathway is distributed across Alpha- and Betaproteobacteria and is especially prevalent within the Rhizobiales order Agrobacterium fabrum
1.1.1.432 physiological function enzyme is part of a pathway for sulfoquinovose degradation. The NAD(P)H-dependent oxidoreductase reduces the 6-oxo-glucose to glucose, enabling entry into primary metabolic pathways Agrobacterium fabrum
1.14.14.181 evolution the sulfoquinovose monooxygenase pathway is distributed across Alpha- and Betaproteobacteria and is especially prevalent within the Rhizobiales order Agrobacterium fabrum
1.14.14.181 physiological function enzyme is part of a pathway for sulfoquinovose degradation. Sulfoquinovose is acted on by flavin mononucleotide-dependent sulfoquinovose monooxygenase, in concert with an NADH-dependent flavin reductase, to release sulfite and 6-oxo-glucose Agrobacterium fabrum