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Literature summary extracted from

  • Malikova, N.P.; Eremeeva, E.V.; Gulnov, D.V.; Natashin, P.V.; Nemtseva, E.V.; Vysotski, E.S.
    Specific activities of hydromedusan Ca2+-regulated photoproteins (2022), Photochem. Photobiol., 98, 275-283 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.24 expression in Escherichia coli BL21 Aequorea victoria
1.13.12.24 expression in Escherichia coli BL21 Obelia longissima
1.13.12.24 expression in Escherichia coli BL21 Obelia geniculata
1.13.12.24 expression in Escherichia coli BL21 Clytia gregaria
1.13.12.24 expression in Escherichia coli BL21 Mitrocoma cellularia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.24 Ca2+ Ca2+-regulated photoprotein Aequorea victoria
1.13.12.24 Ca2+ Ca2+-regulated photoprotein Obelia longissima
1.13.12.24 Ca2+ Ca2+-regulated photoprotein Obelia geniculata
1.13.12.24 Ca2+ Ca2+-regulated photoprotein Clytia gregaria
1.13.12.24 Ca2+ Ca2+-regulated photoprotein Mitrocoma cellularia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ Aequorea victoria
-
 [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ Obelia longissima
-
 [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ Obelia geniculata
-
 [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ Clytia gregaria
-
 [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ Mitrocoma cellularia
-
 [excited state blue fluorescent protein] + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.24 Aequorea victoria P07164
-
-
1.13.12.24 Clytia gregaria Q08121
-
-
1.13.12.24 Mitrocoma cellularia P39047
-
-
1.13.12.24 Obelia geniculata Q8T6Z0
-
-
1.13.12.24 Obelia longissima Q27709
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.24
-
 Aequorea victoria
1.13.12.24
-
 Obelia longissima
1.13.12.24
-
 Obelia geniculata
1.13.12.24
-
 Clytia gregaria
1.13.12.24
-
 Mitrocoma cellularia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
 Aequorea victoria [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
 Obelia longissima [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
 Obelia geniculata [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
 Clytia gregaria [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
 Mitrocoma cellularia [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Aequorea victoria [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Obelia longissima [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Obelia geniculata [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Clytia gregaria [excited state blue fluorescent protein] + CO2
-
 ?
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Mitrocoma cellularia [excited state blue fluorescent protein] + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.24 aequorin
-
 Aequorea victoria
1.13.12.24 clytin
-
 Clytia gregaria
1.13.12.24 mitrocomin
-
 Mitrocoma cellularia
1.13.12.24 obelin
-
 Obelia longissima
1.13.12.24 obelin
-
 Obelia geniculata