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Literature summary extracted from

  • Wang, L.; Ma, X.; Ruan, H.; Chen, Y.; Gao, L.; Lei, T.; Li, Y.; Gui, L.; Guo, L.; Xia, T.; Wang, Y.
    Optimization of the biosynthesis of B-ring ortho-hydroxylated flavonoids using the 4-hydroxyphenylacetate 3-hydroxylase complex (HpaBC) of Escherichia coli (2021), Molecules, 26, 2919 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.5.1.36 synthesis in vivo production of ortho-hydroxylated flavonoids by recombinant Escherichia coli. When HpaC is linked with an S-Tag on the C terminus, the enzyme activity is significantly affected. The optimal culture conditions are a substrate concentration of 80 mg/l, an induction temperature of 28°C, an M9 medium, and a substrate delay time of 6 h after IPTG induction. The efficiency of eriodictyol conversion from recombinant strains fed naringin is up to 57.67. Highest conversion efficiencies for production of catechin and caffeate are 35.2 % and 32.93%, respectively Escherichia coli
1.14.14.9 synthesis in vivo production of ortho-hydroxylated flavonoids by recombinant Escherichia coli. When HpaC is linked with an S-Tag on the C terminus, the enzyme activity is significantly affected. The optimal culture conditions are a substrate concentration of 80 mg/l, an induction temperature of 28°C, an M9 medium, and a substrate delay time of 6 h after IPTG induction. The efficiency of eriodictyol conversion from recombinant strains fed naringin is up to 57.67. Highest conversion efficiencies for production of catechin and caffeate are 35.2 % and 32.93%, respectively Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.36 expression in Escherichia coli Escherichia coli
1.14.14.9 expression in Escherichia coli Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.36 Escherichia coli A0A140NG67
-
-
1.5.1.36 Escherichia coli BL21-DE3 A0A140NG67
-
-
1.14.14.9 Escherichia coli A0A140NG21
-
-
1.14.14.9 Escherichia coli BL21-DE3 A0A140NG21
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.9 naringin + NADH + H+ + O2
-
 Escherichia coli eryodictyol + NADP+ + H2O
-
 ?
1.14.14.9 naringin + NADH + H+ + O2
-
 Escherichia coli BL21-DE3 eryodictyol + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.36 ? x * 58500, SDS-PAGE, recombinant protein Escherichia coli
1.14.14.9 ? x * 58500, SDS-PAGE, recombinant protein Escherichia coli