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Literature summary extracted from

  • Kobayashi, S.; Hira, D.; Yoshida, K.; Toyofuku, M.; Shida, Y.; Ogasawara, W.; Yamaguchi, T.; Araki, N.; Oshiki, M.
    Nitric oxide production from nitrite reduction and hydroxylamine oxidation by copper-containing dissimilatory nitrite reductase (NirK) from the aerobic ammonia-oxidizing archaeon, Nitrososphaera viennensis (2018), Microbes Environ., 33, 428-434 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.1 expressed in Escherichia coli Nitrososphaera viennensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.1 0.287
-
 nitrite pH 6.5, 25°C Nitrososphaera viennensis
1.7.2.1 0.287
-
 nitrite at pH 8.0 and 25°C Nitrososphaera viennensis
1.7.2.6 0.097
-
 hydroxylamine pH 7.5, 30°C Nitrososphaera viennensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.1 copper 2.9 atoms per subunit Nitrososphaera viennensis
1.7.2.1 Cu2+ contains 2.9 atoms per subunit of the recombinant protein Nitrososphaera viennensis
1.7.2.6 copper 2.9 atoms per subunit Nitrososphaera viennensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.1 105000
-
 gel filtration Nitrososphaera viennensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.1 nitrite + ferrocytochrome c + 2 H+ Nitrososphaera viennensis
-
 nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + ferrocytochrome c + 2 H+ Nitrososphaera viennensis JCM19564
-
 nitric oxide + H2O + ferricytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Nitrososphaera viennensis
-
-
-
1.7.2.1 Nitrososphaera viennensis A0A060HKX3
-
-
1.7.2.1 Nitrososphaera viennensis EN76 A0A060HKX3
-
-
1.7.2.1 Nitrososphaera viennensis JCM19564
-
-
-
1.7.2.6 Nitrososphaera viennensis A0A060HKX3 cf. EC 1.7.2.1
-
1.7.2.6 Nitrososphaera viennensis EN76 A0A060HKX3 cf. EC 1.7.2.1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1 His60 Ni Superflow resin column chromatography and Superdex 200 gel filtration Nitrososphaera viennensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 additional information enzyme catalyzes the reduction of NO2- to NO, the oxidation of hydroxylamine (NH2OH) to NO, reaction of EC 1.7.2.6, and the production of N2O from NH2OH and NO2 Nitrososphaera viennensis ?
-
-
1.7.2.1 additional information enzyme catalyzes the reduction of NO2- to NO, the oxidation of hydroxylamine (NH2OH) to NO, reaction of EC 1.7.2.6, and the production of N2O from NH2OH and NO2 Nitrososphaera viennensis EN76 ?
-
-
1.7.2.1 nitrite + ferrocytochrome c + 2 H+
-
 Nitrososphaera viennensis nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + ferrocytochrome c + 2 H+
-
 Nitrososphaera viennensis JCM19564 nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + reduced benzyl viologen
-
 Nitrososphaera viennensis NO + oxidized benzyl viologen
-
 ?
1.7.2.1 nitrite + reduced benzyl viologen
-
 Nitrososphaera viennensis EN76 NO + oxidized benzyl viologen
-
 ?
1.7.2.1 nitrite + reduced benzyl viologen + 2 H+
-
 Nitrososphaera viennensis nitric oxide + H2O + oxidized benzyl viologen
-
 ?
1.7.2.1 nitrite + reduced benzyl viologen + 2 H+
-
 Nitrososphaera viennensis JCM19564 nitric oxide + H2O + oxidized benzyl viologen
-
 ?
1.7.2.1 nitrite + reduced phenazine methosulfate
-
 Nitrososphaera viennensis NO + oxidized phenazine methosulfate
-
 ?
1.7.2.1 nitrite + reduced phenazine methosulfate
-
 Nitrososphaera viennensis EN76 NO + oxidized phenazine methosulfate
-
 ?
1.7.2.6 hydroxylamine + O2
-
 Nitrososphaera viennensis nitrite + H2O
-
 ?
1.7.2.6 hydroxylamine + O2
-
 Nitrososphaera viennensis EN76 nitrite + H2O
-
 ?
1.7.2.6 additional information enzyme catalyzes the reduction of NO2- to NO, the oxidation of hydroxylamine (NH2OH) to NO, reaction of EC 1.7.2.6, and the production of N2O from NH2OH and NO2 Nitrososphaera viennensis ?
-
-
1.7.2.6 additional information enzyme catalyzes the reduction of NO2- to NO, the oxidation of hydroxylamine (NH2OH) to NO, reaction of EC 1.7.2.6, and the production of N2O from NH2OH and NO2 Nitrososphaera viennensis EN76 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.7.2.1 ? x * 40000, SDS-PAGE Nitrososphaera viennensis
1.7.2.1 ? x * 39700, calculated from amino acid sequence Nitrososphaera viennensis

Synonyms

EC Number Synonyms Comment Organism
1.7.2.1 dissimilatory nitrite reductase
-
 Nitrososphaera viennensis
1.7.2.1 NirK
-
 Nitrososphaera viennensis
1.7.2.6 NirK
-
 Nitrososphaera viennensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.1 3.1
-
 nitrite pH 6.5, 25°C Nitrososphaera viennensis
1.7.2.1 3.1
-
 nitrite at pH 8.0 and 25°C Nitrososphaera viennensis
1.7.2.6 0.039
-
 hydroxylamine pH 7.5, 30°C Nitrososphaera viennensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.1 cytochrome c
-
 Nitrososphaera viennensis