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Literature summary extracted from

  • Schachter, H.; Narasimhan, S.; Gleeson, P.; Vella, G.
    Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type (1983), Methods Enzymol., 98, 98-134 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.1 CMP-NeuNAc + Galbeta(1->4)GIcNAc-R Bos taurus
-
 CMP + NeuNAcalpha(2->6)Galbeta(l->4)GlcNAc-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 CDP-ethanolamine-Sepharose column chromatography, ammonium sulfate precipitation, SP-50-Sephadex gel filtration, and Sephadex G-150 gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 colostrum
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.4.3.1 -20°C, pure enzyme in 50% (v/v) glycerol containing 12 mM cacodylate buffer pH 5.3, at least 2 months, no loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-NeuNAc + Galbeta(1-3)GlcNAc
-
 Bos taurus ?
-
 ?
2.4.3.1 CMP-NeuNAc + Galbeta(1->4)GIcNAc-R
-
 Bos taurus CMP + NeuNAcalpha(2->6)Galbeta(l->4)GlcNAc-R
-
 ?
2.4.3.1 CMP-NeuNAc + Galbeta(l->6)GlcNAc
-
 Bos taurus ?
-
 ?
2.4.3.1 CMP-NeuNAc + lactose
-
 Bos taurus ?
-
 ?
2.4.3.1 CMP-NeuNAc + N-acetyllactosamine
-
 Bos taurus ?
-
 ?
2.4.3.1 additional information the enzyme prefers to attach sialic acid to the Gal on the Manalpha(1->3)-arm of the core. Galbeta(l->4)GlcNAc and glycopeptides and glycoproteins with Galbeta(l->4)GlcNAc termini are the only good acceptors. Galbeta(l->3)GalNAc-terminal glycoproteins are not acceptors for enzyme Bos taurus ?
-
-

Subunits

EC Number Subunits Comment Organism
2.4.3.1 ? x * 43000, SDS-PAGE Bos taurus
2.4.3.1 ? x * 56000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.4.3.1 CMP-NeuNAc: Galbeta1-4GlcNAc alpha2-6-sialyltransferase
-
 Bos taurus

General Information

EC Number General Information Comment Organism
2.4.3.1 physiological function the physiological function of the enzyme is almost certainly the synthesis of the NeuNAcalpha(2->6)Galbeta(l->4)GlcNAc-antennae of complex N-glycosyl oligosaccharides Bos taurus