Literature summary extracted from

Uchendu, S.N.; Rafalowski, A.; Cohn, E.F.; Davoren, L.W.; Taylor, E.A.
Anaerobic protein purification and kinetic analysis via oxygen electrode for studying DesB dioxygenase activity and inhibition (2018), J. Vis. Exp., 140, 58307 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.13.11.57	expressed in Escherichia coli BL21(DE3) cells	Sphingobium sp. SYK-6

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.13.11.57	4-nitrocatechol	36.6% inhibition when the inhibitor is present in 5fold excess over substrate	Sphingobium sp. SYK-6

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.13.11.57	0.045	-	gallate	at pH 7.5, temperature not specified in the publication	Sphingobium sp. SYK-6

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.13.11.57	Fe2+	required	Sphingobium sp. SYK-6

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.13.11.57	Sphingobium sp. SYK-6	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.13.11.57	amylose column chromatography	Sphingobium sp. SYK-6

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.13.11.57	gallate + O2	-	Sphingobium sp. SYK-6	(2E,4E)-4-(carboxymethylidene)-2-hydroxypent-2-enedioate	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.13.11.57	?	x * 49220, calculated from amino acid sequence	Sphingobium sp. SYK-6

Synonyms

EC Number	Synonyms	Comment	Organism
1.13.11.57	DesB	-	Sphingobium sp. SYK-6

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.13.11.57	17.8	-	gallate	at pH 7.5, temperature not specified in the publication	Sphingobium sp. SYK-6

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.13.11.57	2.3	-	4-nitrocatechol	at pH 7.5, temperature not specified in the publication	Sphingobium sp. SYK-6

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.13.11.57	398	-	gallate	at pH 7.5, temperature not specified in the publication	Sphingobium sp. SYK-6

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Release 2023.1 (January 2023)