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Literature summary extracted from

  • Schuebel, F.; Rocker, A.; Edelmann, D.; Schessner, J.; Brieke, C.; Meinhart, A.
    3-NADP and 3-NAADP, two metabolites formed by the bacterial type III effector AvrRxo1 (2016), J. Biol. Chem., 291, 22868-22880 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.236 expressed in Escherichia coli BL21(DE3)-RIL cells Xanthomonas oryzae pv. oryzicola

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.236 D193N catalytically impaired mutant Xanthomonas oryzae pv. oryzicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.236 AvrRxo2 potent, mixed inhibitor of the enzyme Xanthomonas oryzae pv. oryzicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.236 1
-
 ATP with NAAD+ or NAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
2.7.1.236 1.2
-
 NAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
2.7.1.236 1.2
-
 NaAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.236 Mg2+ 3 mM used in assay conditions Xanthomonas oryzae pv. oryzicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.236 ATP + NAAD+ Xanthomonas oryzae pv. oryzicola NAAD+ is nicotinic acid adenine dinucleotide ADP + 3'-NAADP+
-
 ?
2.7.1.236 ATP + NAD+ Xanthomonas oryzae pv. oryzicola
-
 ADP + 3'-NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.236 Xanthomonas oryzae pv. oryzicola
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.236 Ni-NTA column chromatography, HiTrap heparin column chromatography, and Superdex 75 gel filtration Xanthomonas oryzae pv. oryzicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.236 ATP + NAAD+ NAAD+ is nicotinic acid adenine dinucleotide Xanthomonas oryzae pv. oryzicola ADP + 3'-NAADP+
-
 ?
2.7.1.236 ATP + NAD+
-
 Xanthomonas oryzae pv. oryzicola ADP + 3'-NADP+
-
 ?
2.7.1.236 GTP + NAD+
-
 Xanthomonas oryzae pv. oryzicola GDP + 3'-NADP+
-
 ?
2.7.1.236 additional information the enzyme accepts neither GDP nor GTP as phosphate acceptors Xanthomonas oryzae pv. oryzicola ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.236 AvrRxo1
-
 Xanthomonas oryzae pv. oryzicola
2.7.1.236 NAD/NAAD kinase
-
 Xanthomonas oryzae pv. oryzicola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.236 270
-
 NaAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
2.7.1.236 340
-
 ATP with NAAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
2.7.1.236 430
-
 NAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
2.7.1.236 460
-
 ATP with NAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.236 225
-
 NaAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
2.7.1.236 340
-
 ATP with NAAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
2.7.1.236 358
-
 NAD+ at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola
2.7.1.236 460
-
 ATP with NAD+ as cosubstrate, at pH 7.5 and 25°C Xanthomonas oryzae pv. oryzicola