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Literature summary extracted from

  • Rai, A.; Klare, J.P.; Reinke, P.Y.A.; Englmaier, F.; Fohrer, J.; Fedorov, R.; Taft, M.H.; Chizhov, I.; Curth, U.; Plettenburg, O.; Manstein, D.J.
    Structural and biochemical characterization of a dye-decolorizing peroxidase from Dictyostelium discoideum (2021), Int. J. Mol. Sci., 22, 6265 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expressed in Escherichia coli Rosetta(DE3)pLysS cells Dictyostelium discoideum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.19 enzyme in the absence of substrate as well as in the presence of potassium cyanide and veratryl alcohol, hanging drop vapor diffusion method, using 2.4 M sodium malonate pH 7.0 Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.19 0.04
-
 Reactive Blue 4 at pH 3.0 and 25°C Dictyostelium discoideum
1.11.1.19 0.166
-
 veratryl alcohol at pH 4.0 and 25°C Dictyostelium discoideum
1.11.1.19 0.37
-
 H2O2 at pH 4.0 and 25°C Dictyostelium discoideum
1.11.1.19 4.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 25°C Dictyostelium discoideum
1.11.1.19 119
-
 pyrogallol at pH 4.0 and 25°C Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Dictyostelium discoideum Q556V8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Dictyostelium discoideum oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.19 guaiacylglycerol-beta-guaiacyl ether + H2O2 + H+
-
 Dictyostelium discoideum oxidized guaiacylglycerol-beta-guaiacyl ether + H2O
-
 ?
1.11.1.19 pyrogallol + H2O2 + H+
-
 Dictyostelium discoideum oxidized pyrogallol + H2O
-
 ?
1.11.1.19 Reactive Blue 4 + H2O2 + H+
-
 Dictyostelium discoideum oxidized Reactive Blue 4 + H2O
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+
-
 Dictyostelium discoideum veratraldehyde + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.19 homodimer
-
 Dictyostelium discoideum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 dye-decolorizing peroxidase
-
 Dictyostelium discoideum
1.11.1.19 DyPA
-
 Dictyostelium discoideum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.19 20 40 the enzyme remains stable from 20-40°C Dictyostelium discoideum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.19 0.000338
-
 veratryl alcohol at pH 4.0 and 25°C Dictyostelium discoideum
1.11.1.19 5.2
-
 Reactive Blue 4 at pH 3.0 and 25°C Dictyostelium discoideum
1.11.1.19 7.9
-
 pyrogallol at pH 4.0 and 25°C Dictyostelium discoideum
1.11.1.19 89.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 25°C Dictyostelium discoideum
1.11.1.19 93.65
-
 H2O2 at pH 4.0 and 25°C Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.19 3
-
 optimum pH for oxidation of Reactive Blue 4 Dictyostelium discoideum
1.11.1.19 4
-
 optimum pH for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pyrogallol and veratryl alcohol Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme
-
 Dictyostelium discoideum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.19 0.00203
-
 veratryl alcohol at pH 4.0 and 25°C Dictyostelium discoideum
1.11.1.19 0.06638
-
 pyrogallol at pH 4.0 and 25°C Dictyostelium discoideum
1.11.1.19 21.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 25°C Dictyostelium discoideum
1.11.1.19 130
-
 Reactive Blue 4 at pH 3.0 and 25°C Dictyostelium discoideum
1.11.1.19 253
-
 H2O2 at pH 4.0 and 25°C Dictyostelium discoideum