Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Czech, L.; Wilcken, S.; Czech, O.; Linne, U.; Brauner, J.; Smits, S.H.J.; Galinski, E.A.; Bremer, E.
    Exploiting substrate promiscuity of ectoine hydroxylase for regio- and stereoselective modification of homoectoine (2019), Front. Microbiol., 10, 2745 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.11.55 synthesis an Escherichia coli cell factory expressing the Pseudomonas stutzeri ectD gene from a synthetic promoter imports homoectoine via the ProU and ProP compatible solute transporters, hydroxylates it, and secretes the formed trans-5-hydroxyhomoectoine, independent from all currently known mechanosensitive channels, into the growth medium Stutzerimonas stutzeri

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.11.55 modeling and docking of different substrates into the crystal structure. The spatial positioning of homoectoine and its hydroxylated derivative in the reaction chamber are super-imposable with that of the 5-hydroxyectoine molecule Sphingopyxis alaskensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.55 0.8
-
 ectoine pH 7.5, 35°C Sphingopyxis alaskensis
1.14.11.55 2.3
-
 ectoine pH 7.5, 35°C Stutzerimonas stutzeri
1.14.11.55 2.6
-
 L-homoectoine pH 7.5, 35°C Stutzerimonas stutzeri
1.14.11.55 3.1
-
 L-homoectoine pH 7.5, 35°C Sphingopyxis alaskensis

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.55 Sphingopyxis alaskensis Q1GNW5
-
-
1.14.11.55 Sphingopyxis alaskensis DSM 13593 Q1GNW5
-
-
1.14.11.55 Stutzerimonas stutzeri A4VFY4 i.e. Pseudomonas stutzeri
-
1.14.11.55 Stutzerimonas stutzeri A1501 A4VFY4 i.e. Pseudomonas stutzeri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.55 ectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis 5-hydroxyectoine + succinate + CO2
-
 ?
1.14.11.55 ectoine + 2-oxoglutarate + O2
-
 Stutzerimonas stutzeri 5-hydroxyectoine + succinate + CO2
-
 ?
1.14.11.55 ectoine + 2-oxoglutarate + O2
-
 Stutzerimonas stutzeri A1501 5-hydroxyectoine + succinate + CO2
-
 ?
1.14.11.55 ectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis DSM 13593 5-hydroxyectoine + succinate + CO2
-
 ?
1.14.11.55 L-homoectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis trans-5-hydroxy-L-homoectoine + succinate + CO2
-
 ?
1.14.11.55 L-homoectoine + 2-oxoglutarate + O2
-
 Stutzerimonas stutzeri trans-5-hydroxy-L-homoectoine + succinate + CO2
-
 ?
1.14.11.55 L-homoectoine + 2-oxoglutarate + O2
-
 Stutzerimonas stutzeri A1501 trans-5-hydroxy-L-homoectoine + succinate + CO2
-
 ?
1.14.11.55 L-homoectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis DSM 13593 trans-5-hydroxy-L-homoectoine + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.55 ? x * 34140, calculated from sequence Sphingopyxis alaskensis
1.14.11.55 ? x * 34180, calculated from sequence and SDS-PAGE Stutzerimonas stutzeri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.11.55 Sphingopyxis alaskensis calculated from sequence
-
 5.5
1.14.11.55 Stutzerimonas stutzeri calculated from sequence
-
 5.5