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Literature summary extracted from

  • Zhang, B.; Gu, H.; Yang, Y.; Bai, H.; Zhao, C.; Si, M.; Su, T.; Shen, X.
    Molecular mechanisms of AhpC in resistance to oxidative stress in Burkholderia thailandensis (2019), Front. Microbiol., 10, 1483 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.26 expressed in Escherichia coli BL21(DE3) cells Burkholderia thailandensis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.26 C171S/C173S inactive Burkholderia thailandensis
1.11.1.26 C57S inactive Burkholderia thailandensis
1.11.1.26 C57S/C171S inactive Burkholderia thailandensis
1.11.1.26 C57S/C173S inactive Burkholderia thailandensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.26 0.0692
-
 cumene hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 0.0978
-
 tert-butyl hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 0.1473
-
 cumene hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 0.1934
-
 H2O2 in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 0.2025
-
 tert-butyl hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 0.3479
-
 H2O2 in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + ROOH Burkholderia thailandensis
-
 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + ROH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.26 Burkholderia thailandensis Q2SWT3
-
-
1.11.1.28 Burkholderia thailandensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.26 Ni-NTA agarose column chromatography Burkholderia thailandensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.26 additional information AhpD directly interacts with the enzyme as an electron donor, and the conserved Cys residues in active site of AhpD are important for enzyme reduction Burkholderia thailandensis ?
-
-
1.11.1.26 NADH + cumene hydroperoxide + H+
-
 Burkholderia thailandensis ?
-
 ?
1.11.1.26 NADH + H2O2 + H+
-
 Burkholderia thailandensis NAD+ + 2 H2O
-
 ?
1.11.1.26 NADH + peroxynitrite + H+
-
 Burkholderia thailandensis ?
-
 ?
1.11.1.26 NADH + tert-butyl hydroperoxide + H+
-
 Burkholderia thailandensis ?
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + ROOH
-
 Burkholderia thailandensis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + ROH
-
 ?
1.11.1.28 additional information AhpD directly interacts with AhpC as an electron donor, and the conserved Cys residues in active site of AhpD are important for enzyme reduction Burkholderia thailandensis ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.11.1.26 AhpC
-
 Burkholderia thailandensis
1.11.1.26 alkyl hydroperoxide reductase subunit C
-
 Burkholderia thailandensis
1.11.1.28 AhpD
-
 Burkholderia thailandensis
1.11.1.28 alkyl hydroperoxide reductase
-
 Burkholderia thailandensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.26 3.6
-
 H2O2 in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 7.1
-
 H2O2 in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 9.5
-
 tert-butyl hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 11.6
-
 cumene hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 26.1
-
 tert-butyl hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 29.7
-
 cumene hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis

General Information

EC Number General Information Comment Organism
1.11.1.26 malfunction enzyme deletion affects the survival rate of Burkholderia thailandensis in response to peroxynitrite Burkholderia thailandensis
1.11.1.26 metabolism the enzyme plays an important role in peroxides and peroxynitrite damage response Burkholderia thailandensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.26 10
-
 H2O2 in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 36
-
 H2O2 in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 47
-
 tert-butyl hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 79
-
 cumene hydroperoxide in the presence of thioredoxin C as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 267
-
 tert-butyl hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis
1.11.1.26 429
-
 cumene hydroperoxide in the presence of AhpD as reducing system, at pH 7.4 and 25°C Burkholderia thailandensis