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Literature summary extracted from

  • VuThi, H.; Jang, S.H.; Lee, C.
    Cloning and characterization of a thermostable glutathione reductase from a psychrophilic Arctic bacterium Sphingomonas sp. (2019), FEMS Microbiol. Lett., 366, fnz218 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.7 expression in Escherichia coli BL21 (DE) Sphingomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+ Sphingomonas sp. the enzyme is an important oxidoreductase that helps maintain redox homeostasis 2 glutathione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Sphingomonas sp. WP_010214957
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+
-
 Sphingomonas sp. 2 glutathione + NADP+
-
 ?
1.8.1.7 glutathione disulfide + NADPH + H+ the enzyme is an important oxidoreductase that helps maintain redox homeostasis Sphingomonas sp. 2 glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer
-
 Sphingomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 glutathione reductase
-
 Sphingomonas sp.
1.8.1.7 SpGR
-
 Sphingomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 60
-
-
 Sphingomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.8.1.7 30 70 30°C: about 50% of maximal activity, 70°C: about 85% of maximal activity Sphingomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.7 50
-
 2 h, 50% residual activity Sphingomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7.5
-
-
 Sphingomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.7 6.5 8 pH 6.5: about 50% of maximal activity, pH 8.0: about 55% of maximal activity Sphingomonas sp.

General Information

EC Number General Information Comment Organism
1.8.1.7 physiological function the enzyme is an important oxidoreductase that helps maintain redox homeostasis. It possesses the characteristics of thermophilic enzymes and functions at colder temperatures, thereby maintaining the reducing power of the Arctic bacterium Sphingomonas sp. PAMC 26621 in a broad range of temperatures Sphingomonas sp.