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Literature summary extracted from

  • Miyazaki, T.; Park, E.
    Crystal structure of the Enterococcusxa0faecalis alpha-N-acetylgalactosaminidase, a member of the glycoside hydrolase family 31 (2020), FEBS Lett., 594, 2282-2293 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.217 apoenzyme and in complex with alpha-N-acetyl-D-galactosaminide, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 3350 and 200 mM ammonium citrate tribasic Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.217 0.136
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide truncated enzyme containing an FN3 domain but not C-terminal domains including a CBM32 domain, at pH 6.0 and 50°C Enterococcus faecalis
3.2.1.217 0.158
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide full-length enzyme, at pH 6.0 and 50°C Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.217 Enterococcus faecalis H7C6Z9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.217 4-nitrophenyl alpha-N-acetyl-D-galactosaminide + H2O
-
 Enterococcus faecalis 4-nitrophenol + N-acetyl-D-galactosamine
-
 ?
3.2.1.217 additional information no activity toward 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl alpha-N-acetyl-D-glucosamide, 4-nitrophenyl alpha-mannoside, and 4-nitrophenyl alpha-D-xyloside Enterococcus faecalis ?
-
-

Synonyms

EC Number Synonyms Comment Organism
3.2.1.217 GH31 alphaGalNAcase
-
 Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.217 50
-
-
 Enterococcus faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.217 4.45
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide truncated enzyme containing an FN3 domain but not C-terminal domains including a CBM32 domain, at pH 6.0 and 50°C Enterococcus faecalis
3.2.1.217 6.54
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide full-length enzyme, at pH 6.0 and 50°C Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.217 6
-
-
 Enterococcus faecalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.217 33
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide truncated enzyme containing an FN3 domain but not C-terminal domains including a CBM32 domain, at pH 6.0 and 50°C Enterococcus faecalis
3.2.1.217 41
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide full-length enzyme, at pH 6.0 and 50°C Enterococcus faecalis