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Literature summary extracted from

  • Washio, T.; Oikawa, T.
    Thermostable and highly specific L-aspartate oxidase from Thermococcus litoralis DSM 5473 cloning, overexpression, and enzymological properties (2018), Extremophiles, 22, 59-71 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.16 expression in Escherichia coli BL21 (DE3) Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.16 7.5
-
 L-aspartate pH 7.0, 70°C Thermococcus litoralis
1.4.3.16 10.2
-
 L-aspartate pH 7.0, 60°C Thermococcus litoralis
1.4.3.16 14.4
-
 L-aspartate pH 7.0, 50°C Thermococcus litoralis
1.4.3.16 21.1
-
 L-aspartate pH 7.0, 37°C Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.16 140000
-
 gel filtration Thermococcus litoralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.16 L-aspartate + O2 Thermococcus litoralis
-
 iminosuccinate + H2O2
-
 ?
1.4.3.16 L-aspartate + O2 Thermococcus litoralis DSM 5473
-
 iminosuccinate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.16 Thermococcus litoralis H3ZLZ5
-
-
1.4.3.16 Thermococcus litoralis DSM 5473 H3ZLZ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.16
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.16 3.86
-
 substrate: L-aspartate, pH 7.0, 70°C Thermococcus litoralis

Storage Stability

EC Number Storage Stability Organism
1.4.3.16 4°C, 96 h, the specific activity of the purified enzyme does not change during the storage period Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.16 L-aspartate + O2
-
 Thermococcus litoralis iminosuccinate + H2O2
-
 ?
1.4.3.16 L-aspartate + O2 highly specific for L-aspartate and does not act on other natural amino acids Thermococcus litoralis iminosuccinate + H2O2
-
 ?
1.4.3.16 L-aspartate + O2
-
 Thermococcus litoralis DSM 5473 iminosuccinate + H2O2
-
 ?
1.4.3.16 L-aspartate + O2 highly specific for L-aspartate and does not act on other natural amino acids Thermococcus litoralis DSM 5473 iminosuccinate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.16 homotrimer 3 * 52000, SDS-PAGE Thermococcus litoralis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.16 Tl-LASPO
-
 Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.16 70
-
 activity increases with temperature up to 70°C Thermococcus litoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.16 80
-
 1 h, stable Thermococcus litoralis
1.4.3.16 85
-
 half-life time: 46 min. 1 h, approximately, 70% of its initial activity remains Thermococcus litoralis
1.4.3.16 90
-
 half-life time: 170 min Thermococcus litoralis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.16 0.7
-
 L-aspartate pH 7.0, 37°C Thermococcus litoralis
1.4.3.16 1.61
-
 L-aspartate pH 7.0, 50°C Thermococcus litoralis
1.4.3.16 2.53
-
 L-aspartate pH 7.0, 60°C Thermococcus litoralis
1.4.3.16 4.26
-
 L-aspartate pH 7.0, 70°C Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.16 8
-
-
 Thermococcus litoralis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.16 6 9
-
 Thermococcus litoralis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.16 5 10.5 highly stable between pH 5.0 and 10.5 Thermococcus litoralis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.16 FAD the enzyme utilized fumaric acid, 2,6-dichlorophenolindophenol, and ferricyanide in addition to FAD as a cofactor under anaerobic conditions Thermococcus litoralis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.16 0.03
-
 L-aspartate pH 7.0, 37°C Thermococcus litoralis
1.4.3.16 0.11
-
 L-aspartate pH 7.0, 50°C Thermococcus litoralis
1.4.3.16 0.25
-
 L-aspartate pH 7.0, 60°C Thermococcus litoralis
1.4.3.16 0.57
-
 L-aspartate pH 7.0, 70°C Thermococcus litoralis