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Literature summary extracted from

  • Roucher, A.; Roussarie, E.; Gauvin, R.M.; Rouhana, J.; Gounel, S.; Stines-Chaumeil, C.; Mano, N.; Backov, R.
    Bilirubin oxidase-based silica macrocellular robust catalyst for on line dyes degradation (2019), Enzyme Microb. Technol., 120, 77-83 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.3.5 additional information the enzyme is used to decolorize recalcitrant dyes Bacillus pumilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.5 expressed in Escherichia coli Origami B (DE3) cells Bacillus pumilus

General Stability

EC Number General Stability Organism
1.3.3.5 after the tenth cycle (2 months of storage), the enzyme retains more than 50% of its initial activity, while for homogeneous solution, the half live of the enzyme is 3 weeks Bacillus pumilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.5 2 bilirubin + O2 Bacillus pumilus
-
 2 biliverdin + H2O
-
 ?
1.3.3.5 2 bilirubin + O2 Bacillus pumilus SAFR-032
-
 2 biliverdin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Bacillus pumilus A8FAG9
-
-
1.3.3.5 Bacillus pumilus SAFR-032 A8FAG9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 2 bilirubin + O2
-
 Bacillus pumilus 2 biliverdin + H2O
-
 ?
1.3.3.5 2 bilirubin + O2
-
 Bacillus pumilus SAFR-032 2 biliverdin + H2O
-
 ?
1.3.3.5 Congo Red + O2 100% decoloration efficiency is reached for Congo Red at pH 4.0 in 8 h Bacillus pumilus ?
-
 ?
1.3.3.5 Congo Red + O2 100% decoloration efficiency is reached for Congo Red at pH 4.0 in 8 h Bacillus pumilus SAFR-032 ?
-
 ?
1.3.3.5 Remazol Brilliant Blue + O2 80% decoloration efficiency is reached for Remazol Brilliant Blue at pH 8.2 in 24 h Bacillus pumilus ?
-
 ?
1.3.3.5 Remazol Brilliant Blue + O2 80% decoloration efficiency is reached for Remazol Brilliant Blue at pH 8.2 in 24 h Bacillus pumilus SAFR-032 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.5 BOD
-
 Bacillus pumilus