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Literature summary extracted from
- Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq (2019), BMC Plant Biol., 19, 195 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.11.9 | expression in Escherichia coli Transetta (DE3) | Albuca bracteata |
| 1.14.20.6 | expression in Escherichia coli Transetta (DE3) | Albuca bracteata |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.9 | 0.03757 | - |
(2S)-naringenin | pH 6.0, 20°C | Albuca bracteata |  |
| 1.14.11.9 | 0.11 | - |
(2S)-naringenin | pH 6.0, 20°C | Albuca bracteata | |
| 1.14.20.6 | 0.47 | 1 | dihydrokaempferol | pH 6.0, 20°C | Albuca bracteata | |
| 1.14.20.6 | 0.526 | - |
dihydrokaempferol | pH 6.0, 20°C | Albuca bracteata | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.9 | Fe2+ | the flavanone 3beta-hydroxylase activity of OcFLS1 and OcFLS2 does not absolutely require iron. However, the presence of sufficient Fe2+ is conducive to successive catalysis of flavanones to flavonols | Albuca bracteata | |
| 1.14.20.6 | Fe2+ | required for activity | Albuca bracteata | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.9 | Albuca bracteata | A0A482LTA5 | - |
- |
| 1.14.11.9 | Albuca bracteata | A0A482LVU5 | - |
- |
| 1.14.20.6 | Albuca bracteata | A0A482LTA5 | - |
- |
| 1.14.20.6 | Albuca bracteata | A0A482LVU5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.11.9 | - |
Albuca bracteata |
| 1.14.20.6 | - |
Albuca bracteata |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.11.9 | bulb | - |
Albuca bracteata | - |
| 1.14.11.9 | flower | - |
Albuca bracteata | - |
| 1.14.11.9 | leaf | high expression | Albuca bracteata | - |
| 1.14.20.6 | bulb | - |
Albuca bracteata | - |
| 1.14.20.6 | flower | - |
Albuca bracteata | - |
| 1.14.20.6 | leaf | high expression | Albuca bracteata | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.9 | (2S)-naringenin + 2-oxoglutarate + O2 | - |
Albuca bracteata | (2R,3R)-dihydrokaempferol + succinate + CO2 | - |
? | |
| 1.14.11.9 | (S)-eriodictyol + 2-oxoglutarate + O2 | - |
Albuca bracteata | dihydroquercetin + succinate + CO2 | - |
? | |
| 1.14.11.9 | (S)-homoeriodictyol + 2-oxoglutarate + O2 | - |
Albuca bracteata | 3'-O-methyltaxifolin + succinate + CO2 | - |
? | |
| 1.14.11.9 | (S)-pinocembrin + 2-oxoglutarate + O2 | - |
Albuca bracteata | pinobanksin + succinate + CO2 | - |
? | |
| 1.14.20.6 | 3'-O-methyltaxifolin + 2-oxoglutarate + O2 | - |
Albuca bracteata | isorhamnetin + succinate + CO2 + H2O | - |
? | |
| 1.14.20.6 | dihydrokaempferol + 2-oxoglutarate + O2 | - |
Albuca bracteata | kaempferol + succinate + CO2 + H2O | - |
? | |
| 1.14.20.6 | dihydroquercetin + 2-oxoglutarate + O2 | - |
Albuca bracteata | quercetin + succinate + CO2 + H2O | - |
? | |
| 1.14.20.6 | pinobanksin + 2-oxoglutarate + O2 | - |
Albuca bracteata | galangin + succinate + CO2 + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.9 | F3H | - |
Albuca bracteata |
| 1.14.11.9 | flavanone 3-hydroxylase | - |
Albuca bracteata |
| 1.14.11.9 | flavanone 3beta-hydroxylase | - |
Albuca bracteata |
| 1.14.11.9 | FLS1 | - |
Albuca bracteata |
| 1.14.11.9 | FLS2 | - |
Albuca bracteata |
| 1.14.20.6 | FLS1 | - |
Albuca bracteata |
| 1.14.20.6 | FLS2 | - |
Albuca bracteata |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.9 | 20 | - |
- |
Albuca bracteata |
| 1.14.20.6 | 20 | - |
- |
Albuca bracteata |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.9 | - |
43 | 0°C: about 80% of maximal activity, 45°C: about 75% of maximal activity | Albuca bracteata |
| 1.14.11.9 | 5 | 50 | 5°C: about 90% of maximal activity, 50°C: about 50% of maximal activity | Albuca bracteata |
| 1.14.20.6 | - |
37 | 0°C: about 60% of maximal activity, 37°C: about 85% of maximal activity | Albuca bracteata |
| 1.14.20.6 | 5 | 37 | 5°C: about 75% of maximal activity, 37°C: about 60% of maximal activity | Albuca bracteata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.9 | 6 | - |
- |
Albuca bracteata |
| 1.14.20.6 | 6 | - |
- |
Albuca bracteata |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.9 | 5 | 9 | pH 5.0: about 40% of maximal activity, pH 9.0: about 50% of maximal activity | Albuca bracteata |
| 1.14.11.9 | 5 | 8 | pH 5.0: about 75% of maximal activity, pH 8.0: about 40% of maximal activity | Albuca bracteata |
| 1.14.20.6 | 5 | 9 | pH 5.0: about 15% of maximal activity, pH 9.0: about 45% of maximal activity | Albuca bracteata |
| 1.14.20.6 | 5 | 8 | pH 5.0: about 70% of maximal activity, pH 8.0: about 40% of maximal activity | Albuca bracteata |
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Release 2023.1 (January 2023)
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