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Literature summary extracted from

  • Fuerst, M.J.L.J.; Boonstra, M.; Bandstra, S.; Fraaije, M.W.
    Stabilization of cyclohexanone monooxygenase by computational and experimental library design (2019), Biotechnol. Bioeng., 116, 2167-2177 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.22 E91Q/A115V/R280Y/N431Y/Q439P/A455V/S534R most stable mutant found, increase in unfolding temperature of 13 K and an approximately 33fold increase in half-life at 30°C Rhodococcus sp. HI-31
1.14.13.22 E91Q/D95H/A115V/Q191M/R280Y/N431Y/Q439P/A455V/S534R increase in melting temperature of 15 K, highly diminished activity Rhodococcus sp. HI-31
1.14.13.22 E91Q/D95H/A115V/R280Y/N431Y/Q439P/A455V/S534R increase in melting temperature of 13 K, highly active Rhodococcus sp. HI-31
1.14.13.22 Q409P/N431Y/A115V/A455V increase in melting temperature of 6.3 K Rhodococcus sp. HI-31

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Rhodococcus sp. HI-31 C0STX7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 cyclohexanone + NADPH + H+ + O2
-
 Rhodococcus sp. HI-31 hexano-6-lactone + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.22 ChnB
-
 Rhodococcus sp. HI-31

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.22 36
-
 melting temperature of wild-type Rhodococcus sp. HI-31
1.14.13.22 48.8
-
 melting temperature of mutant E91Q/D95H/A115V/R280Y/N431Y/Q439P/A455V/S534R Rhodococcus sp. HI-31

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.22 4.9
-
 cyclohexanone mutant E91Q/D95H/A115V/R280Y/N431Y/Q439P/A455V/S534R, pH 7.5, temperature not specified in the publication Rhodococcus sp. HI-31
1.14.13.22 9.2
-
 cyclohexanone wild-type, pH 7.5, temperature not specified in the publication Rhodococcus sp. HI-31