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Literature summary extracted from

  • Miyazaki, T.; Ikegaya, M.; Alonso-Gil, S.
    Structural and mechanistic insights into the substrate specificity and hydrolysis of GH31 alpha-N-acetylgalactosaminidase (2022), Biochimie, 195, 90-99 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.217 mutant enzymes D455A and D455N, hanging drop vapor diffusion method Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.217 D455A the mutant shows 0.34% activity compared to the wild type enzyme Enterococcus faecalis
3.2.1.217 D455N the mutant shows 0.24% activity compared to the wild type enzyme Enterococcus faecalis
3.2.1.217 D508N the mutant shows 0.32% activity compared to the wild type enzyme Enterococcus faecalis
3.2.1.217 I542F the mutant shows 2.2% activity compared to the wild type enzyme Enterococcus faecalis
3.2.1.217 L492R the mutant shows 0.54% activity compared to the wild type enzyme Enterococcus faecalis
3.2.1.217 V456A the mutant shows 34% activity compared to the wild type enzyme Enterococcus faecalis
3.2.1.217 W221N the mutant shows 2.4% activity compared to the wild type enzyme Enterococcus faecalis
3.2.1.217 W570A the mutant shows 1.6% activity compared to the wild type enzyme Enterococcus faecalis
3.2.1.217 Y386A the mutant shows 0.051% activity compared to the wild type enzyme Enterococcus faecalis
3.2.1.217 Y386F the mutant shows 3.4% activity compared to the wild type enzyme Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.217 0.0172
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme D508N, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.0232
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme D455N, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.06
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme Y386F, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.0734
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme D455A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.0794
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme L492R, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.101
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme V456A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.158
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide wild type enzyme, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 2.23
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme I542F, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 2.36
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme Y386A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 3.86
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme W221N, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 4.44
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme W570A, at pH 6.0 and 37°C Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.217 Enterococcus faecalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.217 4-nitrophenyl alpha-N-acetyl-D-galactosaminide + H2O
-
 Enterococcus faecalis 4-nitrophenol + N-acetyl-D-galactosamine
-
 ?
3.2.1.217 BSM + H2O
-
 Enterococcus faecalis N-acetyl-D-galactosamine + ?
-
 ?
3.2.1.217 fetuin + H2O
-
 Enterococcus faecalis N-acetyl-D-galactosamine + ?
-
 ?
3.2.1.217 additional information GalNAcalpha-L-Ser is a poor substrate Enterococcus faecalis ?
-
-
3.2.1.217 mucin + H2O
-
 Enterococcus faecalis N-acetyl-D-galactosamine + ?
-
 ?
3.2.1.217 Tn antigen + H2O low activity Enterococcus faecalis N-acetyl-D-galactosamine + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.217 GH31 alpha-N-acetylgalactosaminidase
-
 Enterococcus faecalis
3.2.1.217 Nag31
-
 Enterococcus faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.217 0.00231
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme D508N, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.00239
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme D455N, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.01
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme D455A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.0175
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme L492R, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.0506
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme Y386A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.0851
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme Y386F, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 1.4
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme V456A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 2.04
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme I542F, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 2.89
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme W570A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 3.79
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme W221N, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 6.54
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide wild type enzyme, at pH 6.0 and 37°C Enterococcus faecalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.217 0.021
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme Y386A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.1
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme D455N, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.13
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme D508N, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.14
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme D455A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.22
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme L492R, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.65
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme W570A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 0.92
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme I542F, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 1
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme W221N, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 1.4
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme Y386F, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 14
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide mutant enzyme V456A, at pH 6.0 and 37°C Enterococcus faecalis
3.2.1.217 41
-
 4-nitrophenyl alpha-N-acetyl-D-galactosaminide wild type enzyme, at pH 6.0 and 37°C Enterococcus faecalis