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Literature summary extracted from

  • Sasoni, N.; Hartman, M.; Guerrero, S.; Iglesias, A.; Arias, D.
    Functional characterization of methionine sulfoxide reductases from Leptospira interrogans (2021), Biochim. Biophys. Acta Proteins Proteom., 1869, 140575 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 expression in Escherichia coli BL21 (DE3) fused to a 6His-MBP Leptospira interrogans
1.8.4.11 expression in Escherichia coli BL21 (DE3) fused to an N-terminal 6Histag Leptospira interrogans
1.8.4.12 expression in Escherichia coli BL21 (DE3) fused to an C-terminal 6Histag Leptospira interrogans

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.11 C18S exhibits lower enzymatic activity than the wild type enzyme Leptospira interrogans
1.8.4.11 C25S exhibits lower enzymatic activity than the wild type enzyme Leptospira interrogans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.11 0.0018
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 0.002
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 0.019
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 0.0249
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 0.0305
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 0.135
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
1.8.4.11 0.28
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
1.8.4.11 0.39
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 0.55
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 1.4
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.12 0.0028
-
 glutaredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
1.8.4.12 0.026
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
1.8.4.12 0.21
-
 N-acetyl-L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
1.8.4.12 1
-
 L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.11 cytoplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans 5737
-
1.8.4.11 periplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans
-
-
1.8.4.12 cytoplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans 5737
-
1.8.4.12 periplasm dual localization, both in cytoplasm and periplasm Leptospira interrogans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.11 21000
-
 calculated from sequence Leptospira interrogans
1.8.4.11 23000
-
 calculated from sequence Leptospira interrogans

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Leptospira interrogans Q72N60 serovar Copenhageni
-
1.8.4.11 Leptospira interrogans Q72UW2 serovar Copenhageni
-
1.8.4.11 Leptospira interrogans Fiocruz L1-130 Q72N60 serovar Copenhageni
-
1.8.4.11 Leptospira interrogans Fiocruz L1-130 Q72UW2 serovar Copenhageni
-
1.8.4.12 Leptospira interrogans Q72NN2 serovar Copenhageni
-
1.8.4.12 Leptospira interrogans Fiocruz L1-130 Q72NN2 serovar Copenhageni
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.11
-
 Leptospira interrogans
1.8.4.12
-
 Leptospira interrogans

Storage Stability

EC Number Storage Stability Organism
1.8.4.11 -80°C, 8 months, no loss of activity Leptospira interrogans
1.8.4.12 -80°C, 8 months, no loss of activity Leptospira interrogans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 L-methionine-(S)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 L-methionine-(S)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans Fiocruz L1-130 L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 N-acetyl-L-methionine-(S)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 N-acetyl-L-methionine-(S)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans Fiocruz L1-130 N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans L-methionine + glutaredoxin disulfide + H2O
-
 ?
1.8.4.12 L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans Fiocruz L1-130 L-methionine + glutaredoxin disulfide + H2O
-
 ?
1.8.4.12 L-methionine-(R)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 L-methionine-(R)-S-oxide + thioredoxin thioredoxin from Leptospira interrogans Leptospira interrogans Fiocruz L1-130 L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 N-acetyl-L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans N-acetyl-L-methionine + glutaredoxin disulfide + H2O
-
 ?
1.8.4.12 N-acetyl-L-methionine-(R)-S-oxide + glutaredoxin glutaredoxin from Homo sapiens Leptospira interrogans Fiocruz L1-130 N-acetyl-L-methionine + glutaredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 LIC_10545
-
 Leptospira interrogans
1.8.4.11 LIC_12978
-
 Leptospira interrogans
1.8.4.11 linmsra1
-
 Leptospira interrogans
1.8.4.11 linmsra2
-
 Leptospira interrogans
1.8.4.11 MsrA1
-
 Leptospira interrogans
1.8.4.11 MSRA2
-
 Leptospira interrogans
1.8.4.12 MsrB
-
 Leptospira interrogans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.11 0.0054
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
1.8.4.11 0.0548
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
1.8.4.11 0.083
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 0.098
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 0.102
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 0.203
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 0.32
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 0.39
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 0.415
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 0.508
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.12 0.0043
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
1.8.4.12 0.0076
-
 L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
1.8.4.12 0.014
-
 N-acetyl-L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
1.8.4.12 0.0145
-
 glutaredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.4.11 0.02
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
1.8.4.11 0.04
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S Leptospira interrogans
1.8.4.11 0.14
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 0.15
-
 N-acetyl-L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 0.25
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 0.71
-
 L-methionine-(S)-S-oxide 30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme Leptospira interrogans
1.8.4.11 40
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 42
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 50
-
 thioredoxin 30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.11 56
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme Leptospira interrogans
1.8.4.12 0.0076
-
 L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
1.8.4.12 0.067
-
 N-acetyl-L-methionine-(R)-S-oxide 30°C, pH 7.0, cosubstrate: glutaredoxin Leptospira interrogans
1.8.4.12 0.17
-
 thioredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans
1.8.4.12 5.2
-
 glutaredoxin 30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide Leptospira interrogans