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Literature summary extracted from

  • Young, L.; Rosell-Hidalgo, A.; Inaoka, D.K.; Xu, F.; Albury, M.; May, B.; Kita, K.; Moore, A.L.
    Kinetic and structural characterisation of the ubiquinol-binding site and oxygen reduction by the trypanosomal alternative oxidase (2020), Biochim. Biophys. Acta Bioenerg., 1861, 148247 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.10.3.11 D100A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 8.8fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
1.10.3.11 D100E kcat/Km-value of the mutant enzyme for ubiquinol-1 is 5.7fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
1.10.3.11 E215A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 34fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
1.10.3.11 E215D kcat/Km-value of the mutant enzyme for ubiquinol-1 is 528fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
1.10.3.11 E215N kcat/Km-value of the mutant enzyme for ubiquinol-1 is 567fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
1.10.3.11 L122A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 34.3fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
1.10.3.11 L212A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 31.2fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
1.10.3.11 R118A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 22.5fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
1.10.3.11 R118K kcat/Km-value of the mutant enzyme for ubiquinol-1 is 19.6fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
1.10.3.11 R96A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 7.9fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
1.10.3.11 R96K kcat/Km-value of the mutant enzyme for ubiquinol-1 is 7.8fold lower than the kcat/Km-value of wild-type enzyme Trypanosoma brucei brucei
1.10.3.11 T219A kcat/Km-value of the mutant enzyme for ubiquinol-1 is 36.4fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei
1.10.3.11 T219S kcat/Km-value of the mutant enzyme for ubiquinol-1 is 89.1fold lower than the kcat/Km-value of wild-type enzyme. No inhibition by ascochlorin, ascofuranone, colletochlorin B, colletochlorin D, octyl-gallate and salicylic hydroxamic acid Trypanosoma brucei brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.11 ascochlorin
-
 Trypanosoma brucei brucei
1.10.3.11 ascofuranone
-
 Trypanosoma brucei brucei
1.10.3.11 colletochlorin B
-
 Trypanosoma brucei brucei
1.10.3.11 colletochlorin D
-
 Trypanosoma brucei brucei
1.10.3.11 octyl-gallate
-
 Trypanosoma brucei brucei
1.10.3.11 salicylic hydroxamic acid
-
 Trypanosoma brucei brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.11 0.0488
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei
1.10.3.11 0.0613
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei
1.10.3.11 0.068
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R118K Trypanosoma brucei brucei
1.10.3.11 0.0782
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei
1.10.3.11 0.0783
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R118A Trypanosoma brucei brucei
1.10.3.11 0.113
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme T219A Trypanosoma brucei brucei
1.10.3.11 0.1217
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei
1.10.3.11 0.1463
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme L122A Trypanosoma brucei brucei
1.10.3.11 0.1697
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme T219S Trypanosoma brucei brucei
1.10.3.11 0.1703
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei
1.10.3.11 0.3374
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215A Trypanosoma brucei brucei
1.10.3.11 0.339
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei
1.10.3.11 0.3473
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215N Trypanosoma brucei brucei
1.10.3.11 0.4295
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215D Trypanosoma brucei brucei
1.10.3.11 5.5
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme E215D Trypanosoma brucei brucei
1.10.3.11 6.2
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei
1.10.3.11 8.1
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei
1.10.3.11 8.5
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme R118A Trypanosoma brucei brucei
1.10.3.11 12
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme E215N Trypanosoma brucei brucei
1.10.3.11 12.9
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme E215A Trypanosoma brucei brucei
1.10.3.11 13.4
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme T219A Trypanosoma brucei brucei
1.10.3.11 13.6
-
 O2 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei
1.10.3.11 14.7
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei
1.10.3.11 14.8
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei
1.10.3.11 15
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme R118K Trypanosoma brucei brucei
1.10.3.11 15.3
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei
1.10.3.11 16.8
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme L122A Trypanosoma brucei brucei
1.10.3.11 17.3
-
 O2 pH 7.4, temperature not specified in the publication, mutant enzyme T219S Trypanosoma brucei brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.11 32000
-
 SDS-PAGE Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.11 2 ubiquinol-1 + O2 Trypanosoma brucei brucei
-
 2 ubiquinone-1 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.11 Trypanosoma brucei brucei Q26710
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.11
-
 Trypanosoma brucei brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.11 2 ubiquinol-1 + O2
-
 Trypanosoma brucei brucei 2 ubiquinone-1 + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.11 trypanosomal alternative oxidase
-
 Trypanosoma brucei brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.10.3.11 0.00000154
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei colletochlorin D
1.10.3.11 0.0000023
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei octyl-gallate
1.10.3.11 0.0000037
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei salicylic hydroxamic acid
1.10.3.11 0.0000044
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei salicylic hydroxamic acid
1.10.3.11 0.0000044
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei salicylic hydroxamic acid
1.10.3.11 0.0000052
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei octyl-gallate
1.10.3.11 0.0000061
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei salicylic hydroxamic acid
1.10.3.11 0.000007
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei ascofuranone
1.10.3.11 0.0000074
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei ascochlorin
1.10.3.11 0.0000075
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei colletochlorin B
1.10.3.11 0.000022
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei salicylic hydroxamic acid
1.10.3.11 0.000033
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei colletochlorin D
1.10.3.11 0.000036
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei colletochlorin B
1.10.3.11 0.000038
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei ascofuranone
1.10.3.11 0.000046
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei octyl-gallate
1.10.3.11 0.000051
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei ascofuranone
1.10.3.11 0.000051
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei colletochlorin D
1.10.3.11 0.000058
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei ascofuranone
1.10.3.11 0.000061
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei colletochlorin D
1.10.3.11 0.000066
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei colletochlorin B
1.10.3.11 0.000081
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei colletochlorin D
1.10.3.11 0.000089
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei ascofuranone
1.10.3.11 0.00009
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei colletochlorin D
1.10.3.11 0.000129
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei ascofuranone
1.10.3.11 0.000137
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei colletochlorin B
1.10.3.11 0.000167
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei ascochlorin
1.10.3.11 0.000203
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei ascochlorin
1.10.3.11 0.000215
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei ascochlorin
1.10.3.11 0.000221
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei ascochlorin
1.10.3.11 0.00023
-
 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei octyl-gallate
1.10.3.11 0.000239
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei colletochlorin B
1.10.3.11 0.000308
-
 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei octyl-gallate
1.10.3.11 0.000316
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei ascochlorin
1.10.3.11 0.00047
-
 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei colletochlorin B
1.10.3.11 0.000862
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei octyl-gallate
1.10.3.11 0.047
-
 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei salicylic hydroxamic acid

General Information

EC Number General Information Comment Organism
1.10.3.11 physiological function the enzyme plays a central role in metabolism through facilitating the turnover of the TCA cycle whilst reducing ROS production Trypanosoma brucei brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.11 1.62
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215N Trypanosoma brucei brucei
1.10.3.11 1.74
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215D Trypanosoma brucei brucei
1.10.3.11 10.3
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme T219S Trypanosoma brucei brucei
1.10.3.11 25.2
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme T219A Trypanosoma brucei brucei
1.10.3.11 26.7
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme L122A Trypanosoma brucei brucei
1.10.3.11 27
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme E215A Trypanosoma brucei brucei
1.10.3.11 29.4
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme L212A Trypanosoma brucei brucei
1.10.3.11 40.8
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R118A Trypanosoma brucei brucei
1.10.3.11 46.6
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R118K Trypanosoma brucei brucei
1.10.3.11 104
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme D100A Trypanosoma brucei brucei
1.10.3.11 116
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R96A Trypanosoma brucei brucei
1.10.3.11 117
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme R96K Trypanosoma brucei brucei
1.10.3.11 162
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, mutant enzyme D100E Trypanosoma brucei brucei
1.10.3.11 918
-
 ubiquinol-1 pH 7.4, temperature not specified in the publication, wild-type enzyme Trypanosoma brucei brucei