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Literature summary extracted from

  • Lencina, A.M.; Koepke, J.; Preu, J.; Muenke, C.; Gennis, R.B.; Michel, H.; Schurig-Briccio, L.A.
    Characterization and X-ray structure of the NADH-dependent coenzyme A disulfide reductase from Thermus thermophilus (2019), Biochim. Biophys. Acta Bioenerg., 1860, 148080 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.14 crystallization by vapor diffusion. X-ray structure is determined at 2.9 A resolution Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.14 0.0025
-
 CoA-disulfide pH 7.5, 42°C Thermus thermophilus
1.8.1.14 0.072
-
 NADH pH 7.5, 42°C Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.14 Thermus thermophilus Q72HK3
-
-
1.8.1.14 Thermus thermophilus ATCC BAA-163 Q72HK3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.14
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.14 5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Thermus thermophilus 2-nitro-5-thiobenzoate + NAD+
-
 ?
1.8.1.14 5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Thermus thermophilus ATCC BAA-163 2-nitro-5-thiobenzoate + NAD+
-
 ?
1.8.1.14 CoA-disulfide + NADH + H+ the specific activity with CoA-disulfide as an electron acceptor is about 5fold higher than with menadione Thermus thermophilus 2 CoA + NAD+
-
 ?
1.8.1.14 CoA-disulfide + NADH + H+ the specific activity with CoA-disulfide as an electron acceptor is about 5fold higher than with menadione Thermus thermophilus ATCC BAA-163 2 CoA + NAD+
-
 ?
1.8.1.14 menadione + NADH + H+
-
 Thermus thermophilus ? + NAD+
-
 ?
1.8.1.14 menadione + NADH + H+
-
 Thermus thermophilus ATCC BAA-163 ? + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.14 homodimer
-
 Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.8.1.14 CoADR
-
 Thermus thermophilus
1.8.1.14 coenzyme A-disulfide reductase
-
 Thermus thermophilus
1.8.1.14 NADH-dependent coenzyme A disulfide reductase
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.14 5
-
 NADH pH 7.5, 42°C Thermus thermophilus
1.8.1.14 5
-
 CoA-disulfide pH 7.5, 42°C Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.14 FAD
-
 Thermus thermophilus
1.8.1.14 NADH the enzyme exclusively oxidizes NADH Thermus thermophilus