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Literature summary extracted from

  • Kim, S.T.; Huh, W.K.; Kim, J.Y.; Hwang, S.W.; Kang, S.O.
    D-arabinose dehydrogenase and biosynthesis of erythroascorbic acid in Candida albicans (1996), Biochim. Biophys. Acta, 1297, 1-8 .
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.427 prolonged storage at 4°C, freezing and thawing, and lyophilisation of enzyme solutions result in significant losses of the enzyme activity Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.427 4-chloromercuribenzoic acid 75% residual activity at 1 mM Candida albicans
1.1.1.427 Cu2+ 69% residual activity at 1 mM Candida albicans
1.1.1.427 Fe2+ 37% residual activity at 1 mM Candida albicans
1.1.1.427 Hg2+ complete inhibition at 1 mM Candida albicans
1.1.1.427 Mg2+ 85% residual activity at 1 mM Candida albicans
1.1.1.427 Mn2+ 87% residual activity at 1 mM Candida albicans
1.1.1.427 additional information not inhibited by EDTA and dithiothreitol Candida albicans
1.1.1.427 N-ethylmaleimide 54% residual activity at 1 mM Candida albicans
1.1.1.427 NADPH NADPH inhibits the enzyme activity competitively with respect to NADP+ Candida albicans
1.1.1.427 Zn2+ 48% residual activity at 1 mM Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.427 0.0446
-
 NADP+ with D-arabinose as cosubstrate, at pH 8.0 and 30°C Candida albicans
1.1.1.427 28.9
-
 L-fucose at pH 8.0 and 30°C Candida albicans
1.1.1.427 29.2
-
 D-arabinose at pH 8.0 and 30°C Candida albicans
1.1.1.427 37.1
-
 L-Xylose at pH 8.0 and 30°C Candida albicans
1.1.1.427 91.3
-
 L-galactose at pH 8.0 and 30°C Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.427 cytosol
-
 Candida albicans 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.427 additional information not influenced by Ca2+ Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.427 41000
-
 gel filtration Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.427 Candida albicans
-
-
-
1.1.1.427 Candida albicans ATCC 10231
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.427 ammonium sulfate precipitation, DEAE-Sepharose A-50, Sephacryl S-200, Cibacron blue and phenyl-Sepharose CL-4B chromatographies Candida albicans

Storage Stability

EC Number Storage Stability Organism
1.1.1.427 -20°C, 20% (v/v) glycerol, at least 2 months, no loss of activity Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.427 D-arabinose + NADP+
-
 Candida albicans D-arabinono-1,4-1actone + NADPH + H+
-
 ?
1.1.1.427 D-arabinose + NADP+
-
 Candida albicans ATCC 10231 D-arabinono-1,4-1actone + NADPH + H+
-
 ?
1.1.1.427 L-fucose + NADP+
-
 Candida albicans ?
-
 ?
1.1.1.427 L-fucose + NADP+
-
 Candida albicans ATCC 10231 ?
-
 ?
1.1.1.427 L-galactose + NADP+
-
 Candida albicans ?
-
 ?
1.1.1.427 L-galactose + NADP+
-
 Candida albicans ATCC 10231 ?
-
 ?
1.1.1.427 L-xylose + NADP+
-
 Candida albicans ?
-
 ?
1.1.1.427 L-xylose + NADP+
-
 Candida albicans ATCC 10231 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.427 monomer 1 * 42000, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.427 D-arabinose dehydrogenase
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.427 30
-
-
 Candida albicans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.427 35 50 the enzyme loses 50% of its activity at 35°C for 60 min and is completely inactivated at 50°C in 30 min Candida albicans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.427 53.2
-
 L-galactose at pH 8.0 and 30°C Candida albicans
1.1.1.427 63.4
-
 L-Xylose at pH 8.0 and 30°C Candida albicans
1.1.1.427 68.2
-
 D-arabinose at pH 8.0 and 30°C Candida albicans
1.1.1.427 69.5
-
 L-fucose at pH 8.0 and 30°C Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.427 8
-
-
 Candida albicans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.427 4.5 7.5 the enzyme is relatively stable from pH 4.5 to 7.5 after 24 h incubation Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.427 NADP+
-
 Candida albicans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.427 Candida albicans isoelectric focusing
-
 5

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.427 0.0786
-
 at pH 8.0 and 30°C Candida albicans NADPH

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.427 0.58
-
 L-galactose at pH 8.0 and 30°C Candida albicans
1.1.1.427 1.71
-
 L-Xylose at pH 8.0 and 30°C Candida albicans
1.1.1.427 2.34
-
 D-arabinose at pH 8.0 and 30°C Candida albicans
1.1.1.427 2.4
-
 L-fucose at pH 8.0 and 30°C Candida albicans