Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reis, R.A.G.; Salvi, F.; Williams, I.; Gadda, G.
    Kinetic investigation of a presumed nitronate monooxygenase from Pseudomonas aeruginosa PAO1 establishes a new class of NAD(P)H quinone reductases (2019), Biochemistry, 58, 2594-2607 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.5 expressed in Escherichia coli Rosetta(DE3)pLysS cells Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.5 additional information when NADH is used instead of NADPH, substrate inhibition is not observed Pseudomonas aeruginosa
1.6.5.5 NADPH substrate inhibition is observed at NADPH concentrations of more than 0.05 mM Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.5 0.012
-
 NADPH with 0.06 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.016
-
 NADPH with 0.08 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.074
-
 5-hydroxy-1,4-naphthoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.1
-
 1,4-Naphthoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.23
-
 2-methyl-1,4-naphthoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.23
-
 NADH with 0.08 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.24
-
 NADH with 0.06 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.37
-
 1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.5
-
 2,3-dimethoxy-5-methyl-1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.5
-
 2,3,4,6-tetramethyl-1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 2.5
-
 2,6-dimethoxy-1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.5 Pseudomonas aeruginosa Q9I5R1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.5 HiTrap column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.5 1,4-benzoquinone + NADPH + H+
-
 Pseudomonas aeruginosa 1,4-benzoquinol + NADP+
-
 ?
1.6.5.5 1,4-naphthoquinone + NADPH + H+
-
 Pseudomonas aeruginosa 1,4-naphthoquinol + NADP+
-
 ?
1.6.5.5 2,3,4,6-tetramethyl-1,4-benzoquinone + NADPH + H+
-
 Pseudomonas aeruginosa 2,3,4,6-tetramethyl-1,4-benzoquinol + NADP+
-
 ?
1.6.5.5 2,3-dimethoxy-5-methyl-1,4-benzoquinone + NADPH + H+
-
 Pseudomonas aeruginosa 2,3-dimethoxy-5-methyl-1,4-benzoquinol + NADP+
-
 ?
1.6.5.5 2,6-dichlorophenolindophenol + NADH + H+ the enzyme is 25times more specific for NADPH as a substrate than for NADH Pseudomonas aeruginosa reduced 2,6-dichlorophenolindophenol + NAD+
-
 ?
1.6.5.5 2,6-dichlorophenolindophenol + NADPH + H+ the enzyme is 25times more specific for NADPH as a substrate than for NADH Pseudomonas aeruginosa reduced 2,6-dichlorophenolindophenol + NADP+
-
 ?
1.6.5.5 2,6-dimethoxy-1,4-benzoquinone + NADPH + H+
-
 Pseudomonas aeruginosa 2,6-dimethoxy-1,4-benzoquinol + NADP+
-
 ?
1.6.5.5 2-methyl-1,4-naphthoquinone + NADPH + H+
-
 Pseudomonas aeruginosa 2-methyl-1,4-naphthoquinol + NADP+
-
 ?
1.6.5.5 5-hydroxy-1,4-naphthoquinone + NADPH + H+
-
 Pseudomonas aeruginosa 5-hydroxy-1,4-naphthoquinol + NADP+
-
 ?
1.6.5.5 additional information the enzyme does not exhibit nitronate monooxygenase activity (no activity with propionate 3-nitronate, 3-nitropropionate, nitroethane, 2-nitropropane, ethylnitronate, or propyl-2-nitronate) and does not turn over with methyl red, consistent with a lack of azoreductase activity Pseudomonas aeruginosa ?
-
-
1.6.5.5 O2 + NADH + H+ the enzyme displays negligible NAD(P)H:oxidase activity Pseudomonas aeruginosa ?
-
 ?
1.6.5.5 O2 + NADPH + H+ the enzyme displays negligible NAD(P)H:oxidase activity Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.5 homodimer 2 * 72000, analytical ultracentrifugation Pseudomonas aeruginosa
1.6.5.5 homodimer 2 * 72200, calculated from amino acid sequence Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.6.5.5 NAD(P)H:quinone reductase
-
 Pseudomonas aeruginosa
1.6.5.5 PA0660
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.5 0.01
-
 NADH with O2 cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.12
-
 NADPH with O2 cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 12
-
 NADH with 0.08 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 13
-
 NADH with 0.06 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 15
-
 2,3,4,6-tetramethyl-1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 15
-
 NADPH with 0.06 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 16
-
 NADPH with 0.08 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 41
-
 2,6-dimethoxy-1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 63
-
 2-methyl-1,4-naphthoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 66
-
 2,3-dimethoxy-5-methyl-1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 80
-
 1,4-Naphthoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 82
-
 5-hydroxy-1,4-naphthoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 87
-
 1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 90
-
 1,4-benzoquinone at pH 7.5 and 25°C Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.5 FMN
-
 Pseudomonas aeruginosa
1.6.5.5 NADH the enzyme can be reduced with NADPH or NADH with a marked preference for NADPH Pseudomonas aeruginosa
1.6.5.5 NADPH the enzyme can be reduced with NADPH or NADH with a marked preference for NADPH Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.5.5 0.17
-
 NADPH with 0.08 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 0.2
-
 NADPH with 0.06 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6.5.5 16
-
 2,6-dimethoxy-1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 30
-
 2,3,4,6-tetramethyl-1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 50
-
 NADH with 0.06 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 50
-
 NADH with 0.08 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 130
-
 2,3-dimethoxy-5-methyl-1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 250
-
 1,4-benzoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 300
-
 2-methyl-1,4-naphthoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 1000
-
 NADPH with 0.08 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 1100
-
 5-hydroxy-1,4-naphthoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 1250
-
 1,4-Naphthoquinone with NADPH as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa
1.6.5.5 1300
-
 NADPH with 0.06 mM 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.5 and 25°C Pseudomonas aeruginosa