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Literature summary extracted from

  • Yao, X.; Lv, Y.; Yu, H.; Cao, H.; Wang, L.; Wen, B.; Gu, T.; Wang, F.; Sun, L.; Xin, F.
    Site-directed mutagenesis of coenzyme-independent carotenoid oxygenase CSO2 to enhance the enzymatic synthesis of vanillin (2020), Appl. Microbiol. Biotechnol., 104, 3897-3907 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.65 glycerol 10% (v/v) glycerol slightly (1.2fold) stimulates vanillin production Caulobacter segnis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.65 expressed in Escherichia coli T7 Express cells Caulobacter segnis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.65 A49P the catalytic efficiency of the mutant is 1.6fold that of the wild type enzyme. The mutant exhibits better thermostability than that of wild type. Under temperatures lower than 30°C, mutant A49P retains almost all the activities within the shorter incubation time (less than 5 h). The mutant also shows better performance even at 35°C Caulobacter segnis
1.13.11.65 I234A the mutant shows strongly decreased catalytic efficiency compared to the wild type enzyme Caulobacter segnis
1.13.11.65 Q390A the catalytic efficiency of the mutant is 1.64fold that of the wild type enzyme. The mutant exhibits better thermostability than that of wild type Caulobacter segnis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.65 Triton X-100 0.1% (v/v) Triton X-100 significantly inhibits vanillin production (to less than 40%) Caulobacter segnis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.65 Fe2+ 0.6 mM used in assay conditions Caulobacter segnis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.65 4-vinylguaiacol + O2 Caulobacter segnis
-
 vanillin
-
 ?
1.13.11.65 4-vinylguaiacol + O2 Caulobacter segnis ATCC 21756
-
 vanillin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.65 Caulobacter segnis D5VGA0
-
-
1.13.11.65 Caulobacter segnis ATCC 21756 D5VGA0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.65 Ni-NTA column chromatography and Superdex 200 gel filtration Caulobacter segnis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.65 4-vinylguaiacol + O2
-
 Caulobacter segnis vanillin
-
 ?
1.13.11.65 4-vinylguaiacol + O2
-
 Caulobacter segnis ATCC 21756 vanillin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.65 ? x * 55000, SDS-PAGE Caulobacter segnis

Synonyms

EC Number Synonyms Comment Organism
1.13.11.65 carotenoid cleavage oxygenase
-
 Caulobacter segnis
1.13.11.65 CSO2
-
 Caulobacter segnis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.65 10 15
-
 Caulobacter segnis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.65 5 30 more than 80% activity between 5 and 30°C. Less than 10% of the maximum activity is retained when the temperature is higher than 30°C Caulobacter segnis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.65 15 35 the enzyme retains about 70-90% activity after approximately 3 h at 15-23°C. At 30°C, the enzyme maintains about 50% activity after 3 h incubation. The enzyme is inactivated after about 3 h at 35°C Caulobacter segnis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.65 7.5
-
-
 Caulobacter segnis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.65 6 10 more than 80% activity between pH 6.0 and 10.0. Less than 10% of the maximum activity is retained when the pH is lower than 6.0 Caulobacter segnis