Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Karakus, Y.; Goc, G.; Balci, S.; Yorke, B.; Trinh, C.; McPherson, M.; Pearson, A.
    Identification of the site of oxidase substrate binding in Scytalidium thermophilum catalase (2018), Acta Crystallogr. Sect. D, 74, 979-985 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.6 hanging-drop vapor diffusion method. Crystal structure of the complex of the catalase with the classical catalase inhibitor 3-amino-1,2,4-triazole is determined at 1.95 A resolution Mycothermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.6 E316F kcat/Km is 2fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
1.11.1.6 E316H kcat/Km is 2.6fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
1.11.1.6 H246W kcat/Km is 5.3fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
1.11.1.6 I313F slight increase in kcat/Km as compared to kcat/Km of wild-type enzyme Mycothermus thermophilus
1.11.1.6 I314F kcat/Km is 5.2fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
1.11.1.6 L321A kcat/Km is 1.2fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
1.11.1.6 V536A kcat/Km is 1.6fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
1.11.1.6 V536W kcat/Km is 5.1fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 10
-
 H2O2 pH 7.0, 60°C, mutant enzyme I313F Mycothermus thermophilus
1.11.1.6 10
-
 H2O2 pH 7.0, 60°C, wild-type enzyme Mycothermus thermophilus
1.11.1.6 11
-
 H2O2 pH 7.0, 60°C, mutant enzyme L321A Mycothermus thermophilus
1.11.1.6 20
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316F Mycothermus thermophilus
1.11.1.6 33
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316H Mycothermus thermophilus
1.11.1.6 40
-
 H2O2 pH 7.0, 60°C, mutant enzyme H246W Mycothermus thermophilus
1.11.1.6 50
-
 H2O2 pH 7.0, 60°C, mutant enzyme I314F Mycothermus thermophilus
1.11.1.6 67
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536A Mycothermus thermophilus
1.11.1.6 600
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536W Mycothermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 2 H2O2 Mycothermus thermophilus
-
 O2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Mycothermus thermophilus M4GGR7
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 0.033
-
 pH 7.0, 60°C, mutant enzyme H246W Mycothermus thermophilus
1.11.1.6 0.085
-
 pH 7.0, 60°C, mutant enzyme V536W Mycothermus thermophilus
1.11.1.6 0.092
-
 pH 7.0, 60°C, mutant enzyme I314F Mycothermus thermophilus
1.11.1.6 0.107
-
 pH 7.0, 60°C, mutant enzyme L321A Mycothermus thermophilus
1.11.1.6 0.163
-
 pH 7.0, 60°C, mutant enzyme E316H Mycothermus thermophilus
1.11.1.6 0.173
-
 pH 7.0, 60°C, mutant enzyme E316F Mycothermus thermophilus
1.11.1.6 0.213
-
 pH 7.0, 60°C, wild-type enzyme Mycothermus thermophilus
1.11.1.6 0.232
-
 pH 7.0, 60°C, mutant enzyme I313F Mycothermus thermophilus
1.11.1.6 0.236
-
 pH 7.0, 60°C, mutant enzyme V536A Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 2 H2O2
-
 Mycothermus thermophilus O2 + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 homotetramer
-
 Mycothermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.6 152000
-
 H2O2 pH 7.0, 60°C, mutant enzyme H246W Mycothermus thermophilus
1.11.1.6 194000
-
 H2O2 pH 7.0, 60°C, mutant enzyme I314F Mycothermus thermophilus
1.11.1.6 196000
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316F Mycothermus thermophilus
1.11.1.6 203410
-
 H2O2 pH 7.0, 60°C, wild-type enzyme Mycothermus thermophilus
1.11.1.6 205000
-
 H2O2 pH 7.0, 60°C, mutant enzyme I313F Mycothermus thermophilus
1.11.1.6 261000
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316H Mycothermus thermophilus
1.11.1.6 274000
-
 H2O2 pH 7.0, 60°C, mutant enzyme L321A Mycothermus thermophilus
1.11.1.6 860500
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536A Mycothermus thermophilus
1.11.1.6 2402000
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536W Mycothermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme the homotetramer contains a heme d in each active site Mycothermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.6 3800
-
 H2O2 pH 7.0, 60°C, mutant enzyme H246W Mycothermus thermophilus
1.11.1.6 3900
-
 H2O2 pH 7.0, 60°C, mutant enzyme I314F Mycothermus thermophilus
1.11.1.6 4000
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536W Mycothermus thermophilus
1.11.1.6 7900
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316H Mycothermus thermophilus
1.11.1.6 9800
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316F Mycothermus thermophilus
1.11.1.6 12900
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536A Mycothermus thermophilus
1.11.1.6 20300
-
 H2O2 pH 7.0, 60°C, wild-type enzyme Mycothermus thermophilus
1.11.1.6 20500
-
 H2O2 pH 7.0, 60°C, mutant enzyme I313F Mycothermus thermophilus
1.11.1.6 24300
-
 H2O2 pH 7.0, 60°C, mutant enzyme L321A Mycothermus thermophilus