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Literature summary extracted from

  • Tagliani, A.; Rossi, J.; Marchand, C.H.; De Mia, M.; Tedesco, D.; Gurrieri, L.; Meloni, M.; Falini, G.; Trost, P.; Lemaire, S.D.; Fermani, S.; Zaffagnini, M.
    Structural and functional insights into nitrosoglutathione reductase from Chlamydomonas reinhardtii (2021), Redox Biol., 38, 101806 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.284 expression in Escherichia coli Chlamydomonas reinhardtii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.284 structure of GSNOR1 of both apo- and holoform, to 1.8 A and 2.3 A resolution, respectively Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 methyl methanethiosulfonate complete inactivation Chlamydomonas reinhardtii
1.1.1.284 additional information not inhibitory: N-ethylmaleimide Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.0143
-
 NADH pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
1.1.1.284 0.0249
-
 S-nitrosoglutathione pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.284 Zn2+ the catalytic domain contains two zinc ions. One zinc ion (Zn402) may have a structural role and it is coordinated with a tetrahedral geometry by cysteine residues Cys100, 103, 106 and 114 in both apo- and holoforms . The second zinc ion (Zn401) lies in the active site and has a catalytic role as a Lewis acid, activating the functional group of the substrate Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 96000
-
 gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284 recombinant protein, purified by Ni2+ affinity chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 additional information no substrate: glutathione disulfide Chlamydomonas reinhardtii ?
-
-
1.1.1.284 S-hydroxymethylglutathione + NAD+
-
 Chlamydomonas reinhardtii S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 S-nitrosoglutathione + NADH + H+
-
 Chlamydomonas reinhardtii GSH + NAD+ + NO
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer 2 * 40000, SDS-PAGE, 2 * 41500, MALDI-TOF Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.284 GSNOR1
-
 Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.284 25 50 30 min, no loss of activity Chlamydomonas reinhardtii
1.1.1.284 65
-
 rapid inactivation above Chlamydomonas reinhardtii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.284 26.6
-
 S-nitrosoglutathione pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
1.1.1.284 27
-
 NADH pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 additional information almost no activity with NADPH Chlamydomonas reinhardtii
1.1.1.284 NADH
-
 Chlamydomonas reinhardtii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.284 1070
-
 S-nitrosoglutathione pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii
1.1.1.284 1860
-
 NADH pH 7.9, temperature not specified in the publication Chlamydomonas reinhardtii