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Literature summary extracted from

  • Timm, S.; Florian, A.; Jahnke, K.; Nunes-Nesi, A.; Fernie, A.R.; Bauwe, H.
    The hydroxypyruvate-reducing system in Arabidopsis multiple enzymes for the same end (2011), Plant Physiol., 155, 694-705 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.81 gene HPR3, At1g12550, genetic structure and RT-PCR expression analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.81 additional information deletion of HPR3 by T-DNA insertion mutagenesis resulting in slightly altered leaf concentrations of the photorespiratory intermediates HP, glycerate, and glycine, indicating a disrupted photorespiratory flux, but not in visible alteration of the phenotype. The combined deletion of HPR1, HPR2, and HPR3 causes increased growth retardation, decreased photochemical efficiency, and reduced oxygen-dependent gas exchange in comparison with the hpr1xhpr2 double mutant. Generation of two independent T-DNA insertion lines for the gene At1g12550, i.e. hpr3-1 and hpr3-2 Arabidopsis thaliana
1.1.1.81 additional information generation of Arabidopsis thaliana HPR1 knockout mutants, which grow nearly normally in ambient air with moderate photoperiods and show only minor changes in photosynthetic and metabolic parameters under these conditions. The additional deletion of the cytosolic HPR2 distinctly elevates the oxygen sensitivity, but this hpr1xhpr2 double mutant can still survive long-term exposure to ambient air Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.81 oxalate strong inhibition Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.81 chloroplast
-
 Arabidopsis thaliana 9507
-
1.1.1.81 cytosol
-
 Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.81 hydroxypyruvate + NADPH + H+ Arabidopsis thaliana
-
 D-glycerate + NADP+
-
 r
1.1.1.81 hydroxypyruvate + NADPH + H+ Arabidopsis thaliana Col-0
-
 D-glycerate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.81 Arabidopsis thaliana Q9CA90
-
-
1.1.1.81 Arabidopsis thaliana Q9LE33
-
-
1.1.1.81 Arabidopsis thaliana Col-0 Q9CA90
-
-
1.1.1.81 Arabidopsis thaliana Col-0 Q9LE33
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.81 hydroxypyruvate + NADPH + H+
-
 Arabidopsis thaliana D-glycerate + NADP+
-
 r
1.1.1.81 hydroxypyruvate + NADPH + H+
-
 Arabidopsis thaliana Col-0 D-glycerate + NADP+
-
 r
1.1.1.81 additional information the enzyme also has glyoxylate reductase (NADP+) activity (EC 1.1.1.79) Arabidopsis thaliana ?
-
-
1.1.1.81 additional information the enzyme also has glyoxylate reductase (NADP+) activity (EC 1.1.1.79) Arabidopsis thaliana Col-0 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.81 At1g12550
-
 Arabidopsis thaliana
1.1.1.81 HPR2
-
 Arabidopsis thaliana
1.1.1.81 HPR3
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.81 NADP+
-
 Arabidopsis thaliana
1.1.1.81 NADPH
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.1.1.81 malfunction deletion of HPR3 results in slightly altered leaf concentrations of the photorespiratory intermediates HP, glycerate, and glycine, indicating a disrupted photorespiratory flux, but not in visible alteration of the phenotype. The combined deletion of HPR1, HPR2, and HPR3 causes increased growth retardation, decreased photochemical efficiency, and reduced oxygen-dependent gas exchange in comparison with the hpr1xhpr2 double mutant. HPR mutants show impaired growth and contain less chlorophyll, phenotypes, detailed overview Arabidopsis thaliana
1.1.1.81 malfunction deletion of photorespiratory enzymes typically leads to a strong air sensitivity of the respective mutants, which can be fully recovered by elevated-CO2 conditions. While this is a distinctive feature of most photorespiratory mutants, Arabidopsis thaliana HPR1 knockout mutants grow nearly normally in ambient air with moderate photoperiods and show only minor changes in photosynthetic and metabolic parameters under these conditions. The additional deletion of the cytosolic HPR2 distinctly elevates the oxygen sensitivity, but this hpr1xhpr2 double mutant can still survive long-term exposure to ambient air Arabidopsis thaliana
1.1.1.81 physiological function HPR3 is the third enzyme in Arabidopsis (Arabidopsis thaliana), which also reduces 4-hydroxypyruvate (HP) to glycerate and shows even more activity with glyoxylate, a more upstream intermediate of the photorespiratory cycle. In silico analysis and proteomic studies target HPR3 to the chloroplast, the enzyme might provide a compensatory bypass for the reduction of HP and glyoxylate within this compartment Arabidopsis thaliana