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Literature summary extracted from

  • Akita, H.; Nakamichi, Y.; Morita, T.; Matsushika, A.
    Identification and functional characterization of NAD(P)+ -dependent meso-diaminopimelate dehydrogenase from Numidum massiliense (2020), MicrobiologyOpen, 9, e1059 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.16 expression in Escherichia coli Numidum massiliense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.16 0.302
-
 NADP+ pH 10.5, temperature not specified in the publication Numidum massiliense
1.4.1.16 3.34
-
 meso-2,6-diaminoheptanedioate pH 10.5, temperature not specified in the publication Numidum massiliense
1.4.1.16 890
-
 NAD+ pH 10.5, temperature not specified in the publication Numidum massiliense

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Numidum massiliense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.16 recombinant protein Numidum massiliense

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.16 701
-
 pH 10.5, temperature not specified in the publication Numidum massiliense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 3-methyl-2-oxobutanoate + NH3 + NADPH + H+
-
 Numidum massiliense ?
-
 ?
1.4.1.16 4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
-
 Numidum massiliense ?
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Numidum massiliense L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Numidum massiliense L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?
1.4.1.16 pyruvate + NH3 + NADPH + H+
-
 Numidum massiliense D-alanine + NADP+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.16 70
-
 30 min, complete loss of activity Numidum massiliense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.16 638
-
 meso-2,6-diaminoheptanedioate pH 10.5, temperature not specified in the publication Numidum massiliense
1.4.1.16 792
-
 NADP+ pH 10.5, temperature not specified in the publication Numidum massiliense
1.4.1.16 8933
-
 NAD+ pH 10.5, temperature not specified in the publication Numidum massiliense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.16 9
-
 optimum for reductive amination, 50°C Numidum massiliense
1.4.1.16 10.5
-
 optimum for oxidative deamination, 50°C Numidum massiliense

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.16 5.5 13 more than 85% of maximum activity Numidum massiliense

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.16 NAD+ NADP+ is 2.5fold preferred over NAD+ Numidum massiliense
1.4.1.16 NADP+ NADP+ is 2.5fold preferred over NAD+ Numidum massiliense

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.16 192
-
 meso-2,6-diaminoheptanedioate pH 10.5, temperature not specified in the publication Numidum massiliense
1.4.1.16 1003
-
 NAD+ pH 10.5, temperature not specified in the publication Numidum massiliense
1.4.1.16 2617
-
 NADP+ pH 10.5, temperature not specified in the publication Numidum massiliense