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Literature summary extracted from

  • Choi, S.; Seo, Y.; Lim, H.; Nam, S.; Kim, G.
    Molecular cloning and overexpression of phytoene desaturase (CrtI) from Paracoccus haeundaensis (2018), Microbiol. Biotechnol. Lett., 46, 145-153 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.31 gene crtI, DNA and amino acid sequence determination and analysis, recombinant enzyme expression in Escherichia coli strain BL21(DE3) Paracoccus haeundaensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.31 additional information
-
 additional information kinetics Paracoccus haeundaensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.99.31 cytoplasm the phytoene desaturase is only expressed as water-soluble enzyme and found in the cytoplasm Paracoccus haeundaensis 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.31 15-cis-phytoene + FAD Paracoccus haeundaensis
-
 all-trans-phytofluene + FADH2
-
 ?
1.3.99.31 all-trans-neurosporene + FAD Paracoccus haeundaensis
-
 all-trans-lycopene + FADH2
-
 ?
1.3.99.31 all-trans-phytofluene + FAD Paracoccus haeundaensis
-
 all-trans-zeta-carotene + FADH2
-
 ?
1.3.99.31 all-trans-zeta-carotene + FAD Paracoccus haeundaensis
-
 all-trans-neurosporene + FADH2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.31 Paracoccus haeundaensis Q24K61
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.31 15-cis-phytoene + FAD
-
 Paracoccus haeundaensis all-trans-phytofluene + FADH2
-
 ?
1.3.99.31 all-trans-neurosporene + FAD
-
 Paracoccus haeundaensis all-trans-lycopene + FADH2
-
 ?
1.3.99.31 all-trans-phytofluene + FAD
-
 Paracoccus haeundaensis all-trans-zeta-carotene + FADH2
-
 ?
1.3.99.31 all-trans-zeta-carotene + FAD
-
 Paracoccus haeundaensis all-trans-neurosporene + FADH2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.31 ? x * 55000, recombinant enzyme, SDS-PAGE Paracoccus haeundaensis
1.3.99.31 More the secondary structure of phytoene desaturase from Paracoccus haeundaensis may contain 18 beta-sheet structures, and 18 alpha-helices and random coil structures Paracoccus haeundaensis

Synonyms

EC Number Synonyms Comment Organism
1.3.99.31 CrtI
-
 Paracoccus haeundaensis
1.3.99.31 phytoene desaturase
-
 Paracoccus haeundaensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.99.31 25
-
 assay at Paracoccus haeundaensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.31 8
-
 assay at Paracoccus haeundaensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.31 FAD required Paracoccus haeundaensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.3.99.31 Paracoccus haeundaensis sequence calculation
-
 8.8