Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Salomone-Stagni, M.; Bartho, J.D.; Kalita, E.; Rejzek, M.; Field, R.A.; Bellini, D.; Walsh, M.A.; Benini, S.
    Structural and functional analysis of Erwinia amylovora SrlD. The first crystal structure of a sorbitol-6-phosphate 2-dehydrogenase (2018), J. Struct. Biol., 203, 109-119 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.140 expression in Escherichia coli BL21(DE3)Star-pLys cells Erwinia amylovora

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.140 sitting-drop vapor-diffusion at 0°C, the crystal structure is determined at 1.84 A resolution Erwinia amylovora

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.140 additional information conversion of D-sorbitol 6-phosphate into D-fructose 6-phosphate is not inhibited by D-mannose 6-phosphate at inhibitor concentrations as high as 10 mM. Conversion of D-fructose 6-phosphate into D-sorbitol 6-phosphate is not observed with concentrations of D-mannose 6-phosphate as high as 5 mM Erwinia amylovora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.140 0.048
-
 NADH pH 9.0, 22°C Erwinia amylovora
1.1.1.140 1.6
-
 NAD+ pH 9.0, 22°C Erwinia amylovora
1.1.1.140 2
-
 D-fructose 6-phosphate pH 9.0, 22°C Erwinia amylovora
1.1.1.140 9
-
 D-sorbitol 6-phosphate pH 9.0, 22°C Erwinia amylovora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.140 D-sorbitol 6-phosphate + NAD+ Erwinia amylovora in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.140 D-sorbitol 6-phosphate + NAD+ Erwinia amylovora CFBP1430 in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) D-fructose 6-phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.140 Erwinia amylovora D4I194
-
-
1.1.1.140 Erwinia amylovora CFBP1430 D4I194
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.140
-
 Erwinia amylovora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.140 D-fructose 6-phosphate + NADH + H+ the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate Erwinia amylovora D-sorbitol 6-phosphate + NAD+
-
 r
1.1.1.140 D-fructose 6-phosphate + NADH + H+ the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate Erwinia amylovora CFBP1430 D-sorbitol 6-phosphate + NAD+
-
 r
1.1.1.140 D-sorbitol 6-phosphate + NAD+ in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) Erwinia amylovora D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.140 D-sorbitol 6-phosphate + NAD+ the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate Erwinia amylovora D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.140 D-sorbitol 6-phosphate + NAD+ in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) Erwinia amylovora CFBP1430 D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.140 D-sorbitol 6-phosphate + NAD+ the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate Erwinia amylovora CFBP1430 D-fructose 6-phosphate + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.140 SrlD
-
 Erwinia amylovora

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.140 0.56
-
 D-fructose 6-phosphate pH 9.0, 22°C Erwinia amylovora
1.1.1.140 5.6
-
 D-sorbitol 6-phosphate pH 9.0, 22°C Erwinia amylovora

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.140 NAD+ Asp61 drives the specificity for NAD(H) over NADP(H) Erwinia amylovora
1.1.1.140 NADH Asp61 drives the specificity for NAD(H) over NADP(H) Erwinia amylovora

General Information

EC Number General Information Comment Organism
1.1.1.140 metabolism in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) Erwinia amylovora

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.140 0.28
-
 D-fructose 6-phosphate pH 9.0, 22°C Erwinia amylovora
1.1.1.140 0.62
-
 D-sorbitol 6-phosphate pH 9.0, 22°C Erwinia amylovora