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Literature summary extracted from

  • Tsevelkhorloo, M.; Kim, S.H.; Kang, D.K.; Lee, C.R.; Hong, S.K.
    NADP+-dependent dehydrogenase SCO3486 and cycloisomerase SCO3480 key enzymes for 3,6-anhydro-L-galactose catabolism in Streptomyces coelicolor A3(2) (2021), J. Microbiol. Biotechnol., 31, 756-763 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.92 expressed in Escherichia coli ER2566 cells Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.92 Ca2+ about 80% residual activity at 5 mM Streptomyces coelicolor
1.2.1.92 Co2+ about 20% residual activity at 5 mM Streptomyces coelicolor
1.2.1.92 Cu2+ about 45% residual activity at 5 mM Streptomyces coelicolor
1.2.1.92 EDTA
-
 Streptomyces coelicolor
1.2.1.92 K+ about 70% residual activity at 5 mM Streptomyces coelicolor
1.2.1.92 Mg2+ about 85% residual activity at 5 mM Streptomyces coelicolor
1.2.1.92 Mn2+ about 50% residual activity at 5 mM Streptomyces coelicolor
1.2.1.92 Na+ about 90% residual activity at 5 mM Streptomyces coelicolor
1.2.1.92 Ni2+ about 20% residual activity at 5 mM Streptomyces coelicolor
1.2.1.92 Zn2+ about 50% residual activity at 5 mM Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.92 Fe2+ 167% activity at 5 mM Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.92 3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O Streptomyces coelicolor
-
 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
1.2.1.92 3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O Streptomyces coelicolor A3(2)
-
 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.92 Streptomyces coelicolor Q9RKF1
-
-
1.2.1.92 Streptomyces coelicolor A3(2) Q9RKF1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.92 Talon metal affinity column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.92 3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O
-
 Streptomyces coelicolor 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
1.2.1.92 3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O 100% activity Streptomyces coelicolor 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
1.2.1.92 3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O
-
 Streptomyces coelicolor A3(2) 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
1.2.1.92 3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O 100% activity Streptomyces coelicolor A3(2) 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
1.2.1.92 D-fructose + NADP+ + H2O about 55% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
1.2.1.92 D-fructose + NADP+ + H2O about 55% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor A3(2) ?
-
 ?
1.2.1.92 D-galactose + NADP+ + H2O about 50% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
1.2.1.92 D-galactose + NADP+ + H2O about 50% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor A3(2) ?
-
 ?
1.2.1.92 D-glucose + NADP+ + H2O 20% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
1.2.1.92 D-glucose + NADP+ + H2O 20% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor A3(2) ?
-
 ?
1.2.1.92 D-glyceraldehyde + NADP+ + H2O less than 20% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
1.2.1.92 D-lyxose + NADP+ + H2O about 15% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
1.2.1.92 D-ribose + NADP+ + H2O about 45% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
1.2.1.92 L-fucose + NADP+ + H2O 25% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
1.2.1.92 L-glyceraldehyde + NADP+ + H2O less than 10% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
1.2.1.92 L-rhamnose + NADP+ + H2O less than 1% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.92 ? x * 50000, SDS-PAGE Streptomyces coelicolor
1.2.1.92 ? x * 55718, calculated from amino acid sequence Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
1.2.1.92 AHG dehydrogenase
-
 Streptomyces coelicolor
1.2.1.92 SCO3486
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.92 50
-
-
 Streptomyces coelicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.92 40 70 the enzyme maintains above 50% activity between 40 and 70°C Streptomyces coelicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.92 40 50 the enzyme is stable up to 40°C but gradually loses its activity at temperatures above 50°C Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.92 6
-
-
 Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.92 5 8 the enzyme shows 44% and 42% activity at pH 5.0 and 8.0, respectively Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.92 NADP+ dependent on Streptomyces coelicolor