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Literature summary extracted from

  • Davis, I.; Yang, Y.; Wherritt, D.; Liu, A.
    Reassignment of the human aldehyde dehydrogenase ALDH8A1 (ALDH12) to the kynurenine pathway in tryptophan catabolism (2018), J. Biol. Chem., 293, 9594-9603 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.32 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.32 N155A the mutant shows very low activity compared to the wild type enzyme Homo sapiens
1.2.1.32 N155D the mutant shows very low activity compared to the wild type enzyme Homo sapiens
1.2.1.32 N155Q the mutant shows very low activity compared to the wild type enzyme Homo sapiens
1.2.1.32 N169A inactive Homo sapiens
1.2.1.32 N169D inactive Homo sapiens
1.2.1.32 N169Q inactive Homo sapiens
1.2.1.32 R109A the variant has a similar turnover number but approximately 160fold increased Km as compared with wild type enzyme Homo sapiens
1.2.1.32 R451A inactive Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.32 0.00059
-
 2-aminomuconate 6-semialdehyde wild type enzyme, pH and temperature not specified in the publication Homo sapiens
1.2.1.32 0.097
-
 2-aminomuconate 6-semialdehyde mutant enzyme R109A, pH and temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O Homo sapiens
-
 2-aminomuconate + NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.32 Homo sapiens Q9H2A2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.32 Ni-NTA column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.32 0.0000031
-
 mutant enzyme N155Q, pH and temperature not specified in the publication Homo sapiens
1.2.1.32 0.0000074
-
 mutant enzyme N155A, pH and temperature not specified in the publication Homo sapiens
1.2.1.32 0.0000074
-
 mutant enzyme N155D, pH and temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.32 2-aminomuconate 6-semialdehyde + NAD+ + H2O
-
 Homo sapiens 2-aminomuconate + NADH + 2 H+
-
 ?
1.2.1.32 2-hydroxymuconate 6-semialdehyde + NAD+ + H2O
-
 Homo sapiens 2-hydroxymuconate + NADH + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.32 2-aminomuconate semialdehyde dehydrogenase
-
 Homo sapiens
1.2.1.32 2-AMS dehydrogenase
-
 Homo sapiens
1.2.1.32 aldehyde dehydrogenase
-
 Homo sapiens
1.2.1.32 ALDH12
-
 Homo sapiens
1.2.1.32 Aldh8a1
-
 Homo sapiens
1.2.1.32 AMSDH
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.32 0.42
-
 2-aminomuconate 6-semialdehyde wild type enzyme, pH and temperature not specified in the publication Homo sapiens
1.2.1.32 1.06
-
 2-aminomuconate 6-semialdehyde mutant enzyme R109A, pH and temperature not specified in the publication Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.32 NAD+
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.2.1.32 metabolism the enzyme is the aldehyde dehydrogenase of the kynurenine pathway of tryptophan catabolism Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.32 11
-
 2-aminomuconate 6-semialdehyde mutant enzyme R109A, pH and temperature not specified in the publication Homo sapiens
1.2.1.32 710
-
 2-aminomuconate 6-semialdehyde wild type enzyme, pH and temperature not specified in the publication Homo sapiens