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Literature summary extracted from

  • Terauchi, A.M.; Lu, S.-F.; Zaffagnini, M.; Tappa, S.; Hirasawa, M.; Tripathy, J.N.; Knaff, D.B.; Farmer, P.J.; Lemaire, S.D.; Hase, T.; Merchant, S.S.
    Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii (2009), J. Biol. Chem., 284, 25867-25878 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.1 0.0173
-
 nitrite cosubstrate Fdx2, pH 7.5, temperature not specified in the publication Synechocystis sp. PCC 6803
1.7.7.1 0.0255
-
 nitrite cosubstrate Fdx2, pH 7.5, temperature not specified in the publication Chlamydomonas reinhardtii
1.7.7.1 0.029
-
 nitrite cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication Chlamydomonas reinhardtii
1.7.7.1 0.0415
-
 nitrite cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication Synechocystis sp. PCC 6803
1.18.1.2 0.0066
-
 oxidized Fdx2 pH 7.5, 25°C Chlamydomonas reinhardtii
1.18.1.2 0.039
-
 oxidized ferredoxin pH 7.5, 25°C Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.2 chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Chlamydomonas reinhardtii
-
-
-
1.7.7.1 Synechocystis sp. PCC 6803 Q55366
-
-
1.18.1.2 Chlamydomonas reinhardtii P53991
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 nitrite + 6 reduced Fdx2 Fdx2 i.e. Chlamydomonas reinhardtii plant-type isoform ferredoxin 2 Synechocystis sp. PCC 6803 ammonia + 2 H2O + 6 oxidized Fdx2
-
 ?
1.7.7.1 nitrite + 6 reduced Fdx2 Fdx2 i.e. Chlamydomonas reinhardtii plant-type isoform ferredoxin 2. Fdx2 is a more specific electron donor to NiR than ferredoxin Chlamydomonas reinhardtii ammonia + 2 H2O + 6 oxidized Fdx2
-
 ?
1.7.7.1 nitrite + 6 reduced ferredoxin
-
 Chlamydomonas reinhardtii ammonia + 2 H2O + 6 oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + 6 reduced ferredoxin
-
 Synechocystis sp. PCC 6803 ammonia + 2 H2O + 6 oxidized ferredoxin
-
 ?
1.18.1.2 2 oxidized Fdx2 + NADPH + H+ Fdx2 i.e. Chlamydomonas reinhardtii plant-type isoform ferredoxin 2 Chlamydomonas reinhardtii 2 reduced Fdx2 + NADP+
-
 ?
1.18.1.2 2 oxidized ferredoxin + NADPH + H+
-
 Chlamydomonas reinhardtii 2 reduced ferredoxin + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.7.1 NiR
-
 Chlamydomonas reinhardtii
1.7.7.1 NirA
-
 Synechocystis sp. PCC 6803
1.18.1.2 PETH
-
 Chlamydomonas reinhardtii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.7.1 1.35
-
 nitrite cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication Chlamydomonas reinhardtii
1.7.7.1 14.6
-
 nitrite cosubstrate Fdx2, pH 7.5, temperature not specified in the publication Chlamydomonas reinhardtii
1.7.7.1 480
-
 nitrite cosubstrate Fdx2, pH 7.5, temperature not specified in the publication Synechocystis sp. PCC 6803
1.7.7.1 666
-
 nitrite cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication Synechocystis sp. PCC 6803
1.18.1.2 248
-
 oxidized Fdx2 pH 7.5, 25°C Chlamydomonas reinhardtii
1.18.1.2 252
-
 oxidized ferredoxin pH 7.5, 25°C Chlamydomonas reinhardtii

General Information

EC Number General Information Comment Organism
1.7.7.1 physiological function the abundance of NiR polypeptide is increased at least 15fold in nitrate-grown cells Chlamydomonas reinhardtii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.7.1 47
-
 nitrite cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication Chlamydomonas reinhardtii
1.7.7.1 573
-
 nitrite cosubstrate Fdx2, pH 7.5, temperature not specified in the publication Chlamydomonas reinhardtii
1.7.7.1 13900
-
 nitrite cosubstrate ferredoxin, pH 7.5, temperature not specified in the publication Synechocystis sp. PCC 6803
1.7.7.1 27700
-
 nitrite cosubstrate Fdx2, pH 7.5, temperature not specified in the publication Synechocystis sp. PCC 6803
1.18.1.2 6440
-
 oxidized ferredoxin pH 7.5, 25°C Chlamydomonas reinhardtii
1.18.1.2 37700
-
 oxidized Fdx2 pH 7.5, 25°C Chlamydomonas reinhardtii