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Literature summary extracted from

  • Schadt, H.S.; Schadt, S.; Oldach, F.; Suessmuth, R.D.
    2-Amino-2-deoxyisochorismate is a key intermediate in Bacillus subtilis p-aminobenzoic acid biosynthesis (2009), J. Am. Chem. Soc., 131, 3481-3483 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.123 A283I mutant completely lacks activity Bacillus subtilis
2.6.1.123 A283K mutant does not form a covalent adduct with chorismate and shows no activity in enzyme assays Bacillus subtilis
2.6.1.123 A283V mutant shows marginal activity Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.123 0.38
-
 NH3 pH not specified in the publication, temperature not specified in the publication Bacillus subtilis
2.6.1.123 0.42
-
 L-glutamate pH not specified in the publication, temperature not specified in the publication Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.123 Bacillus subtilis P28820 subunit PabB
-
2.6.1.123 Bacillus subtilis 168 P28820 subunit PabB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.123 (2S)-2-amino-4-deoxychorismate + NH3
-
 Bacillus subtilis 4-amino-4-deoxychorismate + NH3
-
 ?
2.6.1.123 (2S)-2-amino-4-deoxychorismate + NH3
-
 Bacillus subtilis 168 4-amino-4-deoxychorismate + NH3
-
 ?
2.6.1.123 2 L-glutamine + 2 H2O
-
 Bacillus subtilis 2 L-glutamate + 2 NH3
-
 ?
2.6.1.123 2 L-glutamine + 2 H2O
-
 Bacillus subtilis 168 2 L-glutamate + 2 NH3
-
 ?
2.6.1.123 chorismate + 2 L-glutamine + H2O
-
 Bacillus subtilis 4-amino-4-deoxychorismate + 2 L-glutamate + ammonia
-
 ?
2.6.1.123 chorismate + 2 L-glutamine + H2O
-
 Bacillus subtilis 168 4-amino-4-deoxychorismate + 2 L-glutamate + ammonia
-
 ?
2.6.1.123 chorismate + NH3
-
 Bacillus subtilis (2S)-2-amino-4-deoxychorismate + H2O
-
 ?
2.6.1.123 chorismate + NH3
-
 Bacillus subtilis 168 (2S)-2-amino-4-deoxychorismate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.123 PabB
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.123 0.004
-
 NH3 pH not specified in the publication, temperature not specified in the publication Bacillus subtilis
2.6.1.123 0.005
-
 L-glutamate pH not specified in the publication, temperature not specified in the publication Bacillus subtilis

General Information

EC Number General Information Comment Organism
2.6.1.123 metabolism the PabA subunit acts successively on two molecules of L-glutamine, hydrolysing each to L-glutamate and ammonia (cf. EC 3.5.1.2, glutaminase). The ammonia molecules are channeled to the active site of PabB, which catalyses the formation of 4-amino-4-deoxychorismate from chorismate in two steps via the intermediate 2-amino-4-deoxychorismate. PabB forms a covalent adduct with chorismate Bacillus subtilis