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Literature summary extracted from

  • Rad, M.; Abdossi, V.; Moradi, P.; Rakhshandehroo, F.; Mehrafarin, A.
    Expression analysis of gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and cardenolide and digitoxin production in digitalis purpurea L. using polyamines and methyl jasmonate as elicitors (2020), J. Agric. Sci. Technol., 22, 1551-1561 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 gene DXR, semi-quantitative RT-PCR enzyme expression analysis Digitalis purpurea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.267 chloroplast
-
 Digitalis purpurea 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Digitalis purpurea
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Digitalis purpurea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.267 callus
-
 Digitalis purpurea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Digitalis purpurea 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 1-deoxy-D-xylulose 5-phosphate reductoisomerase
-
 Digitalis purpurea
1.1.1.267 DXR
-
 Digitalis purpurea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADP+
-
 Digitalis purpurea
1.1.1.267 NADPH
-
 Digitalis purpurea

Expression

EC Number Organism Comment Expression
1.1.1.267 Digitalis purpurea elicitors have significant effects on the expression level of DXR and contents of cardenolide and digitoxin additional information
1.1.1.267 Digitalis purpurea the enzyme is induced by methyljasmonate, synergistically increased by polyamines spermidine, spermine, and putrescine. 100 mg/l spermidine + 0.05 mM methyljasmonate lead to 4.1 and 2.4folds increase of cardenolide and digitoxin compared to the control, respectively. The lowest induction levels of cardenolide and digitoxin are obtained by 0.2 mM methyljasmonate without polyamine up

General Information

EC Number General Information Comment Organism
1.1.1.267 physiological function 1-deoxy-xylulose-5-phosphate isomerase-reductase gene (DXR), catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP), which is the key element for MEP-pathway that supports the main C5 units for the formation of mono and diterpenes. Plastidial MEP pathway is considered as the main important source of precursors for essential plastid isoprenoids Digitalis purpurea