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Literature summary extracted from

  • An, B.; Lan, J.; Deng, X.; Chen, S.; Ouyang, C.; Shi, H.; Yang, J.; Li, Y.
    Silencing of D-lactate dehydrogenase impedes glyoxalase system and leads to methylglyoxal accumulation and growth inhibition in rice (2017), Front. Plant Sci., 8, 2071 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.4
-
 Oryza sativa Indica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.4 0.01102
-
 D-2-hydroxybutyrate cosubstrate: ferrocytochrome c, pH 8.35, 25°C Oryza sativa Indica Group
1.1.2.4 0.0149
-
 D-2-hydroxybutyrate cosubstrate: phenazine methosulfate and 2,6-dichlorophenolindophenol, pH 8.35, 25°C Oryza sativa Indica Group
1.1.2.4 0.0414
-
 (R)-lactate cosubstrate: ferrocytochrome c, pH 8.35, 25°C Oryza sativa Indica Group
1.1.2.4 0.0682
-
 (R)-lactate cosubstrate: phenazine methosulfate and 2,6-dichlorophenolindophenol, pH 8.35, 25°C Oryza sativa Indica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.4 mitochondrion
-
 Oryza sativa Indica Group 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.4 Oryza sativa Indica Group
-
-
-
1.1.2.4 Oryza sativa Indica Group 93-11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.4
-
 Oryza sativa Indica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c
-
 Oryza sativa Indica Group pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.4 (R)-lactate + 2 ferricytochrome c
-
 Oryza sativa Indica Group 93-11 pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.4 (R)-lactate + PMS-DCIP PMS-DCIP3 i.e. phenazine methosulfate and 2,6-dichlorophenolindophenol Oryza sativa Indica Group pyruvate + reduced PMS-DCIP
-
 ?
1.1.2.4 (R)-lactate + PMS-DCIP PMS-DCIP3 i.e. phenazine methosulfate and 2,6-dichlorophenolindophenol Oryza sativa Indica Group 93-11 pyruvate + reduced PMS-DCIP
-
 ?
1.1.2.4 D-2-hydroxybutyrate + PMS-DCIP PMS-DCIP3 i.e. phenazine methosulfate and 2,6-dichlorophenolindophenol Oryza sativa Indica Group 2-oxobutanoate + reduced PMS-DCIP
-
 ?
1.1.2.4 D-2-hydroxybutyrate + PMS-DCIP PMS-DCIP3 i.e. phenazine methosulfate and 2,6-dichlorophenolindophenol Oryza sativa Indica Group 93-11 2-oxobutanoate + reduced PMS-DCIP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.4 cytochromec-dependent D-LDH
-
 Oryza sativa Indica Group
1.1.2.4 Os07g06890
-
 Oryza sativa Indica Group

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.4 35
-
-
 Oryza sativa Indica Group

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.2.4 25 40 25°C: about 75% of maximal activity, 40°C: about 95% of maximal activity Oryza sativa Indica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.4 2.6
-
 (R)-lactate cosubstrate: phenazine methosulfate and 2,6-dichlorophenolindophenol, pH 8.35, 25°C Oryza sativa Indica Group
1.1.2.4 5.77
-
 (R)-lactate cosubstrate: ferrocytochrome c, pH 8.35, 25°C Oryza sativa Indica Group
1.1.2.4 9.58
-
 D-2-hydroxybutyrate cosubstrate: ferrocytochrome c, pH 8.35, 25°C Oryza sativa Indica Group
1.1.2.4 10.03
-
 D-2-hydroxybutyrate cosubstrate: phenazine methosulfate and 2,6-dichlorophenolindophenol, pH 8.35, 25°C Oryza sativa Indica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.4 8.35
-
-
 Oryza sativa Indica Group

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.2.4 7 10 pH 7.0: about 50% of maximal activity, pH 10.0: about 70% of maximal activity Oryza sativa Indica Group

General Information

EC Number General Information Comment Organism
1.1.2.4 malfunction under methylglyoxal treatment, silencing of OsD-LDH in rice results in the accumulation of methylglyoxal and D-lactate, the decrease of reduced glutathione in leaves, and ultimately severe growth inhibition. The silencing of OsD-LDH does not affect the growth under photorespiration conditions Oryza sativa Indica Group
1.1.2.4 metabolism the enzyme participates in methylglyoxal metabolism by affecting the activity of the glyoxalase system and aldoketo reductases Oryza sativa Indica Group

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.2.4 38.1
-
 (R)-lactate cosubstrate: phenazine methosulfate and 2,6-dichlorophenolindophenol, pH 8.35, 25°C Oryza sativa Indica Group
1.1.2.4 139
-
 D-2-hydroxybutyrate cosubstrate: phenazine methosulfate and 2,6-dichlorophenolindophenol, pH 8.35, 25°C Oryza sativa Indica Group
1.1.2.4 675
-
 (R)-lactate cosubstrate: ferrocytochrome c, pH 8.35, 25°C Oryza sativa Indica Group
1.1.2.4 869
-
 D-2-hydroxybutyrate cosubstrate: ferrocytochrome c, pH 8.35, 25°C Oryza sativa Indica Group