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Literature summary extracted from

  • Witt, A.; Pozzi, R.; Diesch, S.; Haedicke, O.; Grammel, H.
    New light on ancient enzymes - in vitro CO2 fixation by pyruvate synthase of Desulfovibrio africanus and Sulfolobus acidocaldarius (2019), FEBS J., 286, 4494-4508 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.7.1 analysis biochemical assay for activity, low-potential electrons are introduced by photochemical reduction of EDTA/deazaflavin and the generated pyruvate is trapped by chemical derivatization with semicarbazide. The product of CO2 fixation can be detected as pyruvate semicarbazone by HPLC-MS Desulfocurvibacter africanus
1.2.7.11 analysis biochemical assay for activity, low-potential electrons are introduced by photochemical reduction of EDTA/deazaflavin and the generated pyruvate is trapped by chemical derivatization with semicarbazide. The product of CO2 fixation can be detected as pyruvate semicarbazone by HPLC-MS Sulfolobus acidocaldarius

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.1 expression in Escherichia coli Desulfocurvibacter africanus
1.2.7.11 expression in Escherichia coli Sulfolobus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Desulfocurvibacter africanus P94692
-
-
1.2.7.11 Sulfolobus acidocaldarius Q4J6I9 and Q4J6I8 Q4J6I9 i.e. alpha subunit, Q4J6I8 i.e. beta subunit KorB
-
1.2.7.11 Sulfolobus acidocaldarius DSM 639 Q4J6I9 and Q4J6I8 Q4J6I9 i.e. alpha subunit, Q4J6I8 i.e. beta subunit KorB
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.1 72
-
 acceptor methyl viologen, pH 9, 40°C Desulfocurvibacter africanus
1.2.7.11 10
-
 acceptor methyl viologen, pH 9, 70°C Sulfolobus acidocaldarius

Storage Stability

EC Number Storage Stability Organism
1.2.7.11 4°C, Tris/HCl (20 mM), pH 8.5, 32 days, no loss of catalytic activity Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 Desulfocurvibacter africanus pyruvate + CoA + 2 oxidized ferredoxin
-
 r
1.2.7.1 acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+
-
 Desulfocurvibacter africanus pyruvate + CoA + 2 oxidized methyl viologen
-
 r
1.2.7.1 additional information enzyme is able to use low-potential ferredoxins of the green sulfur bacterium Chlorobium tepidum and of Sulfolobus acidocaldarius whereas CO2 fixation is not supported by the native ferredoxin of Desulfocurvibacter africanus. Methyl viologen as an artificial electron carrier also allows CO2 fixation Desulfocurvibacter africanus ?
-
-
1.2.7.11 2-oxoglutarate + CoA + oxidized methyl viologen
-
 Sulfolobus acidocaldarius succinoyl-CoA + CO2 + reduced methyl viologen
-
 r
1.2.7.11 2-oxoglutarate + CoA + oxidized methyl viologen
-
 Sulfolobus acidocaldarius DSM 639 succinoyl-CoA + CO2 + reduced methyl viologen
-
 r
1.2.7.11 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 Sulfolobus acidocaldarius pyruvate + CoA + 2 oxidized ferredoxin
-
 r
1.2.7.11 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 Sulfolobus acidocaldarius DSM 639 pyruvate + CoA + 2 oxidized ferredoxin
-
 r
1.2.7.11 acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+
-
 Sulfolobus acidocaldarius pyruvate + CoA + 2 oxidized methyl viologen
-
 r
1.2.7.11 additional information enzyme is able to use low-potential ferredoxins of the green sulfur bacterium Chlorobium tepidum and of Sulfolobus acidocaldarius whereas CO2 fixation is not supported by the native ferredoxin of Desulfocurvibacter africanus. Methyl viologen as an artificial electron carrier also allows CO2 fixation Sulfolobus acidocaldarius ?
-
-
1.2.7.11 additional information enzyme is able to use low-potential ferredoxins of the green sulfur bacterium Chlorobium tepidum and of Sulfolobus acidocaldarius whereas CO2 fixation is not supported by the native ferredoxin of Desulfocurvibacter africanus. Methyl viologen as an artificial electron carrier also allows CO2 fixation Sulfolobus acidocaldarius DSM 639 ?
-
-
1.2.7.11 pyruvate + CoA + 2 oxidized ferredoxin
-
 Sulfolobus acidocaldarius acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 r
1.2.7.11 pyruvate + CoA + 2 oxidized ferredoxin
-
 Sulfolobus acidocaldarius DSM 639 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 r
1.2.7.11 pyruvate + CoA + 2 oxidized methyl viologen
-
 Sulfolobus acidocaldarius acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+
-
 r
1.2.7.11 pyruvate + CoA + 2 oxidized methyl viologen
-
 Sulfolobus acidocaldarius DSM 639 acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.7.1 ? x * 130000, calculated from sequence, x * 128000, SDS-PAGE Desulfocurvibacter africanus
1.2.7.11 ? x * 71000 plus x * 34000, SDS-PAGE Sulfolobus acidocaldarius

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 PFOR
-
 Desulfocurvibacter africanus
1.2.7.11 PFOR
-
 Sulfolobus acidocaldarius
1.2.7.11 Saci_2306
-
 Sulfolobus acidocaldarius
1.2.7.11 Saci_2307
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 50 55
-
 Desulfocurvibacter africanus
1.2.7.11 70
-
-
 Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.1 9
-
-
 Desulfocurvibacter africanus
1.2.7.11 10
-
-
 Sulfolobus acidocaldarius