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Literature summary extracted from
- Isolation and characterisation of dhel II, a DNA helicase from Drosophila melanogaster embryos stimulated by Escherichia coli-type single-stranded-DNA-binding proteins (1996), Eur. J. Biochem., 235, 152-158 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.3 | mitochondria1 single-stranded DNA-binding protein | the addition of Escherichia coli-like single-stranded DNA-binding proteins, in particular the mitochondria1 single-stranded DNA-binding protein, stimulates the unwinding activity of the helicase at least 10fold on substrates greater than 200 nucleotides | Drosophila melanogaster |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.3 | Mg2+ | 1 mM used in assay conditions | Drosophila melanogaster |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.3 | 120000 | - |
gel filtration | Drosophila melanogaster |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.3 | Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.2.3 | MonoQ column chromatography and MonoS column chromatography | Drosophila melanogaster |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.6.2.3 | embryo | - |
Drosophila melanogaster | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.3 | 61-nucleotide DNA | - |
Drosophila melanogaster | ? | - |
? | |
| 5.6.2.3 | additional information | the helicase unwinds DNA 5' to 3'and has a preference for purine triphosphates as cofactors for the unwinding reaction (GTP/dGTP are better substrates than ATP/dATP. CTP/dCTP support the reaction at higher concentrations, while UTP and dTTP can hardly be utilised at all) | Drosophila melanogaster | ? | - |
- |
|
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.6.2.3 | monomer | 1 * 120000, SDS-PAGE | Drosophila melanogaster |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.6.2.3 | dhel 11 | - |
Drosophila melanogaster |
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