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Literature summary extracted from

  • Norvienyeku, J.; Zhong, Z.; Lin, L.; Dang, X.; Chen, M.; Lin, X.; Zhang, H.; Anjago, W.M.; Lin, L.; Abdul, W.; Wang, Z.
    Methylmalonate-semialdehyde dehydrogenase mediated metabolite homeostasis essentially regulate conidiation, polarized germination and pathogenesis in Magnaporthe oryzae (2017), Environ. Microbiol., 19, 4256-4277 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.27 gene MGG_01606, single copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis Pyricularia oryzae

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.27 additional information DELTAMomsdh mutant, constructed by DNA targeted replacement of MoMSDH, produces only few restricted lesion on host tissues, MoMSDH deletion accounts for the drastic reduction in lesion size by rendering the DELTAMomsdh mutants incapable of mobilizing the necessary energy required to effectively colonize host tissues. DELTAMomsdh deletion mutants exhibit higher intracellular level of amino acids Val, Leu, Ile, and of pyridoxine Pyricularia oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.27 acetaldehyde about 10% inhibition at 3 mM Pyricularia oryzae
1.2.1.27 DTT about 40% inhibition at 2 mM Pyricularia oryzae
1.2.1.27 ethanol complete inhibition at 3% v/v Pyricularia oryzae
1.2.1.27 H2O2 about 40% inhibition at 3 mM Pyricularia oryzae
1.2.1.27 malonate about 25% inhibition at 1 mM Pyricularia oryzae
1.2.1.27 NaCl about 40% inhibition at 0.7 mM Pyricularia oryzae
1.2.1.27 pyridoxine complete inhibition at 0.1 mg/ml Pyricularia oryzae
1.2.1.27 SDS about 50% inhibition at 0.01% Pyricularia oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Pyricularia oryzae
-
 propanoyl-CoA + HCO3- + NADH + H+
-
 ?
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Pyricularia oryzae FGSC 8958
-
 propanoyl-CoA + HCO3- + NADH + H+
-
 ?
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Pyricularia oryzae ATCC MYA-4617
-
 propanoyl-CoA + HCO3- + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.27 Pyricularia oryzae G4MTY9 cv. Guy11, Pyricularia oryzae
-
1.2.1.27 Pyricularia oryzae ATCC MYA-4617 G4MTY9 cv. Guy11, Pyricularia oryzae
-
1.2.1.27 Pyricularia oryzae FGSC 8958 G4MTY9 cv. Guy11, Pyricularia oryzae
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.27 conidium
-
 Pyricularia oryzae
-
1.2.1.27 mycelium
-
 Pyricularia oryzae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
 Pyricularia oryzae propanoyl-CoA + HCO3- + NADH + H+
-
 ?
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
 Pyricularia oryzae FGSC 8958 propanoyl-CoA + HCO3- + NADH + H+
-
 ?
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
 Pyricularia oryzae ATCC MYA-4617 propanoyl-CoA + HCO3- + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.27 More MoMsdh domain architecture, overview Pyricularia oryzae

Synonyms

EC Number Synonyms Comment Organism
1.2.1.27 methylmalonate-semialdehyde dehydrogenase
-
 Pyricularia oryzae
1.2.1.27 MGG_01606
-
 Pyricularia oryzae
1.2.1.27 MoMSDH
-
 Pyricularia oryzae
1.2.1.27 MSDH
-
 Pyricularia oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.27 NAD+
-
 Pyricularia oryzae

Expression

EC Number Organism Comment Expression
1.2.1.27 Pyricularia oryzae during infection, e.g. of susceptible rice cv. CO39 and seedlings of the barley cv. Golden Promise, two ALDH genes (MoMSDH and MoDEL) are exclusively upregulated at 48-hpi with MoMSDH showing about 5.8fold increase in expression pattern during the growth of the rice blast fungus in host tissue. The remaining eight Magnaporthe oryzae ALDH genes show low and unaltered expression pattern at all stages of infection up

General Information

EC Number General Information Comment Organism
1.2.1.27 evolution evolutionary lineage history of Msdh across kingdoms, MSDH belongs to the aldehyde dehydrogenase (ALDH) superfamily of genes which are highly conserved and widely distributed in almost all organisms across kingdoms. In vivo expression pattern of Magnaporthe oryzae specific ALDH genes during host-pathogen interaction, overview Pyricularia oryzae
1.2.1.27 malfunction MoMSDH deletion adversely affected the development of conidiophore and, as a result, conidiophore population as well as the number of conidia per conidiophore produced by the DELTAMomsdh deletion mutants are significantly reduced Pyricularia oryzae
1.2.1.27 metabolism contribution of the numerous ALDH genes to the fungal pathogenesis, 16 ALDH genes in Magnaporthe oryzae are idetified involved in infection and pathogenesis Pyricularia oryzae
1.2.1.27 physiological function enzyme MoMsdh exerts minimal influence on the development of vegetative hyphae, but is involved in the regulation of conidiogenesis and conidiophoregenesis in Magnaporthe oryzae. Conidiation constitutes a pivotal developmental stage in fungal life-cycle and represents one of the most durable organs that promote fungal survival and facilitate their efficient dissemination. MoMsdh is involved in the regulating conditions necessary for promoting asexual reproductive development in Magnaporthe oryzae. MoMsdh is essential for pathogenicity of the organism. MoMsdh crucially regulates intracellular level of small branched-chain amino acids and appressoria mediating signalling molecules. MoMsdh specifically controls the localization of Spitzenkoerper in the conidium to ensure polarized speciation of germ tube. MoMsdh enhances membrane integrity by detoxifying alcohol and pyridoxine derived reactive osmolytes Pyricularia oryzae